树脂固定化β-半乳糖苷酶制备低聚半乳糖  

Galactooligosaccharides production by β-galactosidase immobilized onto resins

作　　者：费俊杰[1] 李冰冰[1] 陈勇[1] 应汉杰[1] 

机构地区：[1]南京工业大学生物与制药工程学院国家生化工程技术研究中心,南京211800

出　　处：《生物加工过程》2015年第4期17-22,共6页Chinese Journal of Bioprocess Engineering

基　　金：国家杰出青年基金(21025625);国家高技术研究发展计划(863计划)(2012AA021203);国家支撑计划(2012BAIG4001);国家自然科学基金青年基金(21106070);长江学者和创新发展计划(PCSIRT);江苏省高校优势学科建设工程;江苏自然科学基金(SBK201150207)

摘　　要：以树脂为载体研究β-半乳糖苷酶固定化条件,来改善酶性质。以吸附率和回收率最高的离子交换树脂I002为载体,通过先吸附后交联的方法固定β-半乳糖苷酶,优化固定化条件。结果表明:加酶量为51.8 U(以1 g树脂计),固定p H为6.5,温度是25℃,吸附时间12 h,戊二醛体积分数为4%,交联温度是40℃,时间是6 h时,固定化效果最好。获得的固定化酶活可达16.2 U,固定酶回收率为39.1%,得到低聚半乳糖(GOS)的产率为24.2%。该研究为工业化利用固定化乳糖酶连续生产低聚半乳糖提供了技术依据。We used resin as a carrier to immobilize β-galactosidase for the production of galactooligosaccharides. Cation exchange resin with the highest absorption rate and recover rate was selected as the carrier. The immobilization condition was optimized through cross-linking after immobilization of β-galactosidase. The best condition was enzyme load 51. 8 U / g resin,p H 6. 5,25 ℃,time 12 h; and the concentration of glutaraldehyde of 4%（ v / v）; the temperature in cross-linking action was 40 ℃,the time was 6 h. Under the conditions,the activity of immobilized enzyme was 16. 2 U / g resin,the recovery of enzyme fixed was 39. 1%,the galactooligosaccharides（ GOS） production was24. 2%. The method provides technical basis for continuous production of GOS.

关 键 词：Β-半乳糖苷酶 固定化 低聚半乳糖 树脂 

分 类 号：Q814[生物学—生物工程]