高糖状态对脂多糖诱导人牙龈上皮细胞表达炎症因子的影响  

Effects of high glucose on inflammatory cytokine expressions in human gingival epithelial cells stimulated by lipopolysaccharide

作　　者：杨熙[1] 罗世高[2] 轩东英[1] 黄馨[1] 谢宝仪[1] 章锦才[1] 

机构地区：[1]广东省口腔医院.南方医科大学附属口腔医院牙周病科,广东广州510280 [2]四川省人民医院口腔科

出　　处：《广东牙病防治》2015年第7期341-345,共5页Journal of Dental Prevention and Treatment

基　　金：国家自然科学基金面上项目(81371151;81271160);广东省自然科学基金博士科研启动项目(2014A030310405)

摘　　要：目的研究高糖状态对脂多糖(lipopolysaccharide,LPS)诱导人牙龈上皮细胞(human gingival epithelial cells,HGECs)表达炎症因子的影响。方法原代培养HGECs,取第3代细胞分别在含5.5 mmol/L D-葡萄糖(对照组)、含25 mmol/L D-葡萄糖(高糖组)培养基中培养48 h后,加入0μg/m L、1μg/m L、5μg/m L和10μg/m L的LPS,2 h、4 h和8 h时荧光定量逆转录聚合酶链反应检测炎症因子白细胞介素-6(interleukin-6,IL-6)、白细胞介素-8(interleukin-8,IL-8)、白细胞介素-1β(interleukin-1,IL-1β)的mRNA表达,ELISA法检测细胞培养上清液中IL-8的表达。结果在5μg/m L LPS刺激8 h后,高糖组和对照组HGECs炎症因子转录水平IL-6分别为13.20±0.84和8.85±0.53(t=7.60,P=0.002),IL-8分别为14.88±1.54和8.12±0.46(t=7.281,P=0.002),IL-1β分别为1.69±0.19和1.27±0.11(t=3.348,P=0.029),两组比较差异均具有统计学意义(P<0.05);两组细胞培养上清液中IL-8的表达分别为(134.0±10.8)pg/m L和(103.0±11.0)pg/m L,差异具有统计学意义(t=3.480,P=0.025)。结论高糖状态可增强LPS诱导HGECs炎症因子IL-6、IL-8、IL-1β的表达。Objective To investigate the effects of high glucose on expressions of inflammatory cytokines in human gingival epithelial cells （ HGECs） stimulated by lipopolysaccharide （ LPS） .Methods Primary HGECs were cultured in vitro and the 3rd generation cells were harvested.The cells were assigned to high glucose group （25 mmol/L D-glucose） and control group （5.5 mmol/L D-glucose）.Each group was divided into 4 sub-groups after 48 h culture, which were treated with LPS at different concentrations respectively （0 μg/mL, 1 μg/mL, 5 μg/mL and 10 μg/mL）.Real-time quantitative PCR （ RT-PCR ） was applied to detect the expressions of gene [ interleukin-6 （ IL-6 ） , interleukin-1β（IL-1β）, interleukin-8 （IL-8）] levels, and the expressions of IL-8 in cell culture supernatant at 2 h, 4 h and 8 h after LPS stimulation was detected by ELISA.Results After stimulation by 5μg /mL LPS for 8 h, the transcription levels of inflammatory factors in high glucose group were significantly higher than that of control group, showing as IL-6 （13.20 ± 0.84 and 8.85 ±0.53, t=7.60, P=0.002）, IL-8 （14.88 ±1.54 and 8.12 ±0.46, t=7.281, P=0.002）, and IL-1β（1.69 ±0.19 and 1.27 ±0.11, t=3.348, P=0.029）.In addition, the level of IL-8 in culture supernatant of high glucose group was significantly increased compared with control group （134.0 ±10.8 pg/mL and 103.0 ±11.0 pg/mL, t=3.480, P=0.025）.Conclusion High glucose might promote the expressions of inflammatory cytokines in HGECs stimulated by LPS, such as IL-6, IL-8, IL-1β.

关 键 词：牙周炎 糖尿病 牙龈上皮细胞 脂多糖 

分 类 号：R781.4[医药卫生—口腔医学]