响应面优化超声辅助提取唐古特白刺蛋白质的工艺研究  

Optimization of Ultrasound-assisted Extraction of Protein From Nitraria tangutorum Bor by Response Surface Methodology

作　　者：高清雅 赵保堂[1,2] 张继[1,2] 赵庆芳[1] 殷振雄[1,2] 

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070 [2]甘肃省特色农产品高值化工程实验室,甘肃兰州730070

出　　处：《北方园艺》2015年第14期141-147,共7页Northern Horticulture

基　　金：国家科技支撑计划资助项目(2012BAD36B04)

摘　　要：以唐古特白刺为试材,在单因素试验的基础上,选取液料比、超声时间、超声功率和提取温度为影响因子,应用Box-Behnken中心组合设计建立数学模型,以蛋白质得率为响应值,进行响应面分析。结果表明:超声波提取唐古特白刺中蛋白质的最佳工艺条件为超声时间30min,超声温度50℃,液料比为20∶1mL/g,超声功率150W,提取次数2次,在此条件下唐古特白刺蛋白质得率达到(11.87±0.32)%。响应面法建立的数学模型和试验数据相符,优化的唐古特白刺中蛋白质提取工艺方法简便,为进一步开发唐古特白刺蛋白提供了依据。Taking Nitraria tangutorum Bor as test material,based on single factor experiments,solvent-solid ratio,ultrasonic time,supersonic power and ultrasound tissue temperature were selected as influencing factors.The experiment mathematical model was arranged according to Box-Behnken central composite design,for protein yield as response values.The results showed that the optimum extraction conditions were that the ultrasonic time 30 minutes,ultrasonic temperature 50℃,the ratio of liquid to solid 20∶1mL/g,ultrasonic power 150 W.The protein extraction yield was（11.87±0.32）%.The mathematical model and the experimental data were consistented with response surface method;the optimization of the fruit of Nitraria tangutorum Bor protein extraction process was simple,provided the basis for the further development of Nitraria tangutorum Bor protein.

关 键 词：唐古特白刺 蛋白质 响应面法 超声波提取 

分 类 号：S793.9[农业科学—林木遗传育种]