5-羟甲基糠醛对模拟高原大鼠血红蛋白氧结合特性及游泳耐力和空间记忆能力的影响  

Effect of 5-HMF on hemoglobin-oxygen affinity,swimming endurance and spatial memory ability of rats in high altitude simulated environment

作　　者：官立彬[1] 李晓栩[1] 李锦松[2] 杨诚忠 谭小玲[1] 崔宇[1] 高钰琪[3] 黄瑊[1] 

机构地区：[1]第三军医大学高原军事医学系:高原生理学与高原生物学教研室,重庆400038 [2]第三军医大学高原军事医学系高原特殊药物与装备研究室,高原医学教育部重点实验室,全军高原医学重点实验室 [3]广东药学院生命科学与生物制药学院,广州510006

出　　处：《第三军医大学学报》2015年第15期1561-1565,共5页Journal of Third Military Medical University

基　　金：国家重点基础研究发展计划(973计划,2012CB518201);国家自然科学基金面上项目(81370150,30973446)~~

摘　　要：目的探讨血红蛋白变构剂5-羟甲基糠醛[5-(Hydroxymethyl)-2-furfural,5-HMF]对血红蛋白氧结合特性的作用及其对大鼠耐缺氧能力的影响。方法将SD成年雄性大鼠随机分为4组:平原空白组、高原空白组、平原用药组、高原用药组。空白组用生理盐水、用药组以50 mg/kg 5-HMF腹腔给药。高原暴露采用模拟海拔5 000 m低压舱内24 h。各组按原剂量经腹腔重复给药30 min后从腹主动脉采血,测血气及氧平衡曲线。大鼠经过游泳训练后,负重游泳力竭实验检测负重游泳力竭时间。大鼠经过记忆训练后,Morris水迷宫空间探索试验测大鼠空间记忆能力。结果高原空白组负重游泳力竭时间、目标象限大鼠滞留时间、穿越平台位置次数、血氧饱和度、血氧分压等指标,较平原空白组显著降低(P<0.05),高原用药后这些指标显著升高,但是负重游泳力竭时间、目标象限大鼠滞留时间仍低于平原水平。各给药组P50、希尔系数均较相应空白组显著降低(P<0.05)。平原用药组较平原空白组负重游泳力竭时间显著降低(P<0.05),血氧分压显著升高,其余指标无统计学差异(P>0.05)。结论 5-HMF在低氧条件下可增加血红蛋白氧亲和力、血氧饱和度及血氧分压,增强游泳耐力和空间记忆能力。Objective To investigate the effect of 5-（ hydroxymethyl）-2-furfural （5-HMF）, hemoglobin allosteric agent, on the hemoglobin-oxygen affinity and hypoxia resistance capacity. Methods Adult SD male rats were randomly divided into a plain blank group, a high altitude blank group, a plain administration group and a high altitude administration group. 5-HMF was intraperitoneally injected into the SD rats at a dose of 50 mg/kg, and the rats were exposed in a hypoxia chamber for simulating 5 000 m of high altitude environment for 24 h. After 30 min repeated administration, blood was collected from the abdominal aorta to determine blood oxygen saturation （SaO2 ）, partial pressure of carbon dioxide （PaCO2 ）, partial pressure of oxygen （PaO2 ）, HCO3 , and oxygen binging curve. Exhaustion swimming time and spatial memory ability of the trained rats were assayed in the presence or absence of 5-HMF under hypoxia or normoxia. Morris water maze was applied to assay spatial memory ability. Results 5-HMF administration significantly decreased 1）50 and Hill coefficient under hypoxia or normoxia. Under hypoxia, PaCO2, SaO2, PaO2, I-ICO3-, exhaustion time, target quadrant retention time and frequency across the platform were all decreased significantly compared to those under normoxia. These indicators were all increased significantly after 5-HMF administration under hypoxia, but still lower than those under normoxia, except HCO3- and frequency across the platform. After administration, PaO2 was increased, and exhaustion time was decreased under normoxia. Conclusion 5-HMF increases hemoglobin-oxygen affinity, SaO2 and PaO2 under hypoxia, and further ameliorates the swimming endurance and spatial memory ability.

关 键 词：低氧 作业能力 氧亲和力 5-HMF 游泳耐力 空间记忆能力 

分 类 号：R331.141[医药卫生—人体生理学] R364.4[医药卫生—基础医学]