S100A4、SEPT7在儿童神经胶质瘤的表达特点及临床意义  

Expression and clinical significance of S100A4 and SEPT7 in childhood gliomas

作　　者：李毅力[1] 李映良[1] 梁平[1] 翟暄[1] 周渝冬[1] 李禄生[1] 

机构地区：[1]重庆医科大学附属儿童医院神经外科,重庆400014

出　　处：《第三军医大学学报》2015年第16期1673-1676,共4页Journal of Third Military Medical University

摘　　要：目的研究儿童神经胶质瘤中S100A4、SEPT7的表达并比较其表达特点,探讨其在儿童神经胶质瘤中的表达与病理分级间的关系及临床意义。方法采用免疫组化(S-P法)检测10例儿童正常脑组织标本和37例儿童脑神经胶质瘤标本中S100A4、SEPT7的表达,计算其阳性细胞的标记指数和阳性细胞表达率,通过SPSS 17.0分析儿童神经胶质瘤中S100A4、SEPT7的表达和儿童神经胶质瘤病理分级的相关性。结果 S100A4呈棕黄色颗粒散在分布,在细胞质和细胞间质中阳性表达。在儿童神经胶质瘤组织中表达率为72.97%,在儿童正常对照组织中未见明显表达,病理分级与S100A4的阳性表达呈正相关(r=0.800,P<0.05)。SEPT7蛋白在儿童脑神经胶质细胞瘤中的表达主要存在细胞质内,在儿童正常脑组织中的表达为100.00%,在神经胶质瘤组织中表达率为54.05%,病理分级与SEPT7的阳性表达呈负相关(r=-0.674,P<0.05)。S100A4和SEPT7两者在同一级别儿童胶质瘤中的表达呈负相关(r=-0.617,P<0.01)。结论联合检测儿童神经胶质瘤S100A4和SEPT7的蛋白表达对评估儿童胶质瘤的恶性程度有重要的参考意义。Objective To determine the expression of S100A4 and SEPT7 in the glioma tissues of children, and to investigate the relationship of their expression with pathological grades and clinical significance. Mothods Immunohistochemical staining （S-P） assay was employed to simultaneously detect the expression of S100A4 and SEPT7 in 10 tissue samples of normal children brain （autopsy from children died of accidence） and 37 glioma tissues from childhood gliomas （37 children underwent surgical treatment in our department during May 2008 to December 2012）. The labeling index of positive cells and the rate of positive cells were calculated according to the expression of optical density. The correlation of their expression with the pathological grades of childhood glioma was analyzed by SPSS17.0 statistical software. Results S100A4 was expressed as brown granules scattering in the cytoplasm and intercellular substance of childhood gliomas. Its positive expression rate was 72.97% in children gliomas, but no expression was seen in normal brain tissue. There was a positive correlation of the pathological grade with its expression. The expression of SEVI＇7 was mainly localized in the cytoplasm of glioma cells, with a rate of 54.05%. The expression rate was 100.00% in normal brain tissue. Its expression rate was negatively correlated with the pathological grade. The expression of S100A4 was negatively correlated with that of SEPT7 at the same pathological grade of childhood gliomas （r = -0. 617, P 〈 0. 01 ）. Coflclosion Combined detection of the expression levels of protein S100A4 and SEPT7 is of important significance to evaluate the malignancy of childhood glioma.

关 键 词：神经胶质细胞瘤 儿童 S100A4 SEPT7 免疫组织化学 

分 类 号：R730.23[医药卫生—肿瘤] R730.43[医药卫生—临床医学]