蛋白激酶C介导转化生长因子β诱导人口腔鳞癌细胞整合素β6的表达  

Protein Kinase C Mediates Transforming Growth Factorβ-induced integrinβ6 Expression in Human Oral Squamous Cancer Cells

作　　者：谷胤征 邓小玲[1] 李祺福[1] 石松林[1] 陈锡和[2] 许铭炎[1,3] 

机构地区：[1]福建省厦门大学医学院,福建厦门361102 [2]广东省汕头大学医学院 [3]福建省厦门市口腔医院

出　　处：《口腔医学研究》2015年第7期684-687,共4页Journal of Oral Science Research

基　　金：国家自然科学基金项目(编号:81072208;81370160;30900661);福建省医学创新课题项目(2014-CXB-47);中央高校基本科研业务费专项资金资助

摘　　要：目的:研究蛋白激酶C(PKC)是否参与转化生长因子β(TGF-β)诱导的人口腔鳞癌细胞(SAS)整合素β6表达。方法:人口腔鳞癌细胞SAS培养于含10%胎牛血清DMEM培养基中,经TGF-β诱导后,裂解细胞提取总RNA和蛋白质,采用实时荧光定量RT-PCR及蛋白印迹方法分别检测整合素β6mRNA和蛋白表达情况;利用PKC阻断剂研究PKC对整合素β6表达的影响。结果:TGF-β诱导人口腔鳞癌SAS细胞整合素β6mRNA及蛋白表达呈时间依赖性;广谱型PKC抑制剂RO-31-8220呈剂量依赖性阻断TGF-β诱导的SAS细胞整合素β6mRNA和蛋白表达,而Ca2+依赖型PKC抑制剂G6976则没有阻断作用。结论:非Ca2+依赖途径的PKC介导TGF-β诱导人口腔鳞癌细胞SAS细胞整合素β6表达。Objective：To investigate whether protein kinase C（PKC）is involved in the expression of integrinβ6by human oral squamous cancer cells（SAS）after transforming growth factorβ（TGF-β）stimulation.Methods：Human oral squamous cancer cells SAS were cultured with DMEM with 10% fetal bovine serum,after TGF-βstimulation,integrinβ6protein and mRNA levels in the cell lysate were measured by western-blot and quantitative real-time PCR,respectively.Effect of PKC inhibition on integrinβ6expression was investigated by using PKC inhibitors.Results：TGF-βinduced SAS integrinβ6mRNA and protein expression levels in a time-dependent manner.These effects of TGF-βcould be attenuated by the broad spectrum PKC inhibitor RO-31-8220 in a concentration-dependent manner,whereas Ca^2＋-dependent PKC inhibitor G6976had no effect.Conclusion：Ca2＋-independent PKC mediates TGF-β-induced integrinβ6expression in cultured SAS cells.

关 键 词：转化生长因子Β 蛋白激酶 C抑制剂 整合素β6 人口腔鳞癌细胞 

分 类 号：R739.8[医药卫生—肿瘤]