STAT3促进舌鳞癌细胞侵袭与转移的实验研究  

STAT3 Promotes Human Lingual Squamous Cell Carcinoma Invasion and Metastasis

作　　者：赵艳[1] 张壁[1] 陈琦[1] 王贻宁[1] 魏军成 

机构地区：[1]武汉大学口腔医院修复科,湖北武汉430079 [2]华中科技大学同济医学院附属同济医院妇产科

出　　处：《口腔医学研究》2015年第7期688-690,694,共4页Journal of Oral Science Research

基　　金：国家自然科学基金青年项目(编号:81400544)

摘　　要：目的:研究信号转导和转录激活因子3(STAT3)在人舌鳞状细胞癌组织中的表达,探讨STAT3对舌鳞癌细胞侵袭与转移的促进作用。方法:免疫组织化学染色法检测STAT3在舌鳞状细胞癌组织中的表达,利用小分子干扰RNA(siRNA)体外抑制舌鳞癌细胞系Tca-8113中STAT3的表达,运用体外侵袭实验检测细胞侵袭能力的改变。结果:STAT3表达与肿瘤临床分期(P=0.029)及淋巴结转移(P=0.009)密切相关;siRNA抑制STAT3表达后,细胞侵袭能力明显下降。结论:STAT3促进舌鳞癌细胞侵袭与转移,STAT3siRNA可以抑制肿瘤细胞的体外侵袭能力。Objective：To investigate the expression of signal transducers and activators of transcription 3（Stat3）in human lingual squamous cell carcinoma and to evaluate the role of STAT3 for tumor cell invasion and metastasis.Methods：The immunohistochemistry method was used to detect the expression of STAT3 in human lingual squamous cell carcinoma.The human lingual squamous cell carcinoma cell line,Tca-8113 cells were transfected with STAT3 small interfering RNA（siRNA）.Transwell assay was used to study cell invasion.Results：There was a significant correlation between STAT3 expression and stages（P=0.029）and lymph node metastasis（P=0.009）.The specific knockdown of STAT3 by RNA interference strongly inhibited the motile and invasion activity of tumor cells.Conclusion：STAT3promotes human lingual squamous cell carcinoma invasion and metastasis.STAT3 siRNA can inhibit the invasion ability of tumor cells.

关 键 词：舌鳞状细胞癌 信号转导和转录激活因子3 小分子干扰RNA 

分 类 号：R739.86[医药卫生—肿瘤]