参芪泄浊饮对大鼠腺嘌呤肾间质纤维化模型大鼠肾组织MMP-1、TIMP-1基因表达影响随机平行对照研究  

Effects of Shenqi Xiezhuo Decoction on Rat MMP-1,TIMP-1 Expression in Adenine-induced Renal Interstitial Fibrosis Rats

作　　者：王冉[1] 何学红[2] 

机构地区：[1]辽宁中医药大学第一临床学院 [2]辽宁中医药大学附属医院肾内科,沈阳110032

出　　处：《实用中医内科杂志》2015年第7期133-136,共4页Journal of Practical Traditional Chinese Internal Medicine

摘　　要：[目的]研究参芪泄浊饮对腺嘌呤致大鼠慢性肾衰肾组织基质金属蛋白酶1(MMP-1)、基质金属蛋白酶抑制剂1(TIMP-1)基因表达的影响。[方法]SPF级雄性SD大鼠54只,采用Yokozawa法复制慢性肾衰大鼠模型,随机分为参芪泄浊饮高剂量组、参芪泄浊饮常规剂量组、氯沙坦组、模型组,分别予高剂量组、常规剂量组、氯沙坦组以参芪泄浊饮水煎液20m L/kg·d、10m L/kg·d、氯沙坦组9mg/kg·d,模型组与空白组大鼠均予纯净水10m L/kg·d,连续灌胃干预8周,ELISA法测量血肌酐(Scr)、尿素氮(BUN)、丙氨酸转氨酶(ALT);荧光定量PCR方法检测肾组织中MMP-1、TIMP-1。[结果]1Scr、BUN各组大鼠较空白组均有升高(P<0.05),氯沙坦组明显低于模型组(P<0.05);Scr高剂量组大鼠明显低于常规剂量组、氯沙坦组及模型组大鼠(P<0.05),常规剂量组与氯沙坦组亦低于模型组(P<0.05);BUN变化趋势与Scr相同,高剂量组降低不明显(P>0.05)。2MMP-1 m RNA各组均高于空白组(P<0.05),高剂量组显著高于常规剂量组、氯沙坦组及模型组(P<0.05),常规剂量组与氯沙坦组也显著高于模型组(P<0.05);TIMP-1 m RNA各组均高于空白组(P<0.05),高剂量组明显低于常规剂量组、氯沙坦组及模型组(P<0.05),常规剂量组与氯沙坦组明显低于模型组(P<0.05)。[结论]参芪泄浊饮可通过促进MMP-1 m RNA及抑制TIMP-1 m RNA表达,使细胞外基质(extracellular matrix,ECM)的降解增加,堆积减少,进而减缓CRF的进一步发展,保护肾脏。[Objective]To investigate the effects of Shenqi Xiezhuo decoction on matrix metalloproteinase- 1（MMP-1）and tissue inhibitor of matrix metalloproteinase-1（TIMP-1） expression in adenine-induced renal interstitial fibrosis rats.[Methods]SPF male SD rats were 54,the renal failure model of the remaining rats were established by Yokozawa method.All rats were randomly devided into 5 groups： high-dose group,regular-dose group,losartan group,model group and control group.Model group and the control group of rats herein are pure water.After 8 weeks,all rats were collected to analyz by serum biochemical tests and RT-PCR.Results：（1）The level of serum crcatinine of high-dose group was significantly lower than the levels of regular-dose group or losartan group,and there was no significant difference between the latter two groups.（2）The expression of MMP-1 mRNA of high-dose group was more than the regular-dose group or losartan group,and there was no significant difference between the latter two groups.The expression of TIMP-1 mRNA of high-dose group was less than the regular-dose group or losartan group,and there was no significant difference between the latter two groups.（3）There was no difference of the level of ALT between control group and the others.Conclusions： Shenqi Xiezhuo decoction may alleviate deterioration of the renal function by regulating MMP-1 and TIMP-1 balance.

关 键 词：大鼠腺嘌呤肾间质纤维化模型 参芪泄浊饮 腺嘌呤 基质金属蛋白酶-1(MMP-1) 基质金属蛋白酶抑制剂-1(MMP-1) 细胞外基质(ECM) 随机平行对照研究 

分 类 号：R739.45[医药卫生—肿瘤]