超临界CO_2流体萃取及GC-MS联用技术优化提取和分析四叶参挥发油成分  

Supercritical CO_2 fluid extraction and GC-MS spectrometry to optimize the extraction and analysis of volatile oil components from Codonopsis lanceolata

作　　者：高艳霞[1] 苏延友[1] 贾凤娟[2] 李艳玲[1] 苗增民[1] 洒荣波[1] 唐瑜菁[1] 孙继政[1] 

机构地区：[1]泰山医学院,泰安271016 [2]山东农业大学生命科学学院,泰安271018

出　　处：《中国新药杂志》2015年第14期1665-1669,共5页Chinese Journal of New Drugs

基　　金：山东省科技发展计划(2014GSF119024)

摘　　要：目的:利用超临界CO2流体萃取技术及气象色谱-质谱(GC-MS)联用技术提取并分析四叶参挥发油成分。方法:利用超临界CO2流体萃取技术,从四叶参中萃取挥发油并进行工艺优化。用GC-MS技术对其化学成分进行分析,并采用面积归一化法确定其百分含量。结果:四叶参挥发油超临界CO2流体萃取的最佳提取工艺为:萃取温度40℃,萃取压力20 MPa,萃取时间3 h,萃取得率1.21%。利用GC-MS联用技术鉴定出46种化合物,其中组分含量最高的是A-菠菜甾醇15.71%,其次是1,4-Dimethyl-8-isopropylidenetricyclo[5.3.0.0(4,10)]decane 13.64%,再次是反式角鲨烯12.12%,然后是亚油酸甲酯11.45%,D7-菠菜甾醇10.52%,这几个成分占四叶参挥发油总量的63.44%。结论:该方法优化了四叶参挥发油的提取工艺并对其成分进行了定性分析,为四叶参的综合开发利用奠定了基础。Objective： To extract and analyze the chemical components of volatile oil from Codonopsis lanceolata. Methods： Supercritical CO2 fluid extraction（ SFE-CO2） procedure was used as the extraction method and its process was optimized. The volatile oil was separated by GC-MS and determined by normalization method. Results： The best conditions were temperature 40 ℃,pressure 20 MPa,and extraction time 3 h. The yield of volatile oil was 1. 21%. Forty-six volatile compounds were identified from the volatile oil of Codonopsis lanceolata. The main compounds of volatile oil were stigmasta,[7,22-dien-3-ol,（ beta,5 alpha,22 E,24R） ] accounting for15. 71%,1,4-dimethyl-8-isopropylidenetricyclo [5. 3. 0. 0（ 4,10） ] decane for 13. 64%,trans-squalene for12. 12%,9,12-octadecadienoic acid methyl ester for 11. 45%,and stigmast-7-en-3-ol,（ 3 beta,5 alpha,24S）for 10. 52%. These components accounted for 63. 44% of the total volatile oil from Codonopsis lanceolata. Conclusion： The optimized SFE-CO2 method coupled with GC / MS is efficient. This may be a good method for further comprehensive development and utilization of Codonopsis lanceolata.

关 键 词：四叶参 超临界CO2萃取 工艺优化 气相色谱-质谱 定性分析 

分 类 号：R284.2[医药卫生—中药学]