miR-106a在人前列腺癌中的临床意义及生物学机制研究  

Expression and clinical significance of miR-106a in human prostatic carcinoma

作　　者：李金虎[1] 赵晓俊[1] 张江磊[1] 魏雪栋[1] 侯建全[1] 

机构地区：[1]苏州大学附属第一医院泌尿外科,苏州215006

出　　处：《中国免疫学杂志》2015年第7期955-959,共5页Chinese Journal of Immunology

摘　　要：目的:探讨Micro RNA-106a(mi R-106a)在前列腺癌(Prostatic carcinoma,PC)中的表达情况及其临床病理意义。方法:收集2013年10月至2014年10月间我院泌尿外科行手术切除的前列腺癌组织及对应癌旁组织共43例,运用q RTPCR检测mi R-106a在PC组织及癌旁组织中的表达水平,卡方检验分析mi R-106a表达水平与患者临床病理资料间的相关性;免疫组化检测不同mi R-106a含量的前列腺癌组织中mi R-106a下游潜在靶点Mcl-1及MMP2蛋白的表达水平,统计分析Mcl-1、MMP2蛋白与mi R-106a表达间的相关性。采用化学合成的mi R-106a模拟物转染前列腺癌LNCap细胞,采用q RT-PCR及Western blot检测mi R-106a对LNCap细胞中Mcl-1和MMP2 m RNA及蛋白的调控作用。结果:mi R-106a在PC组织中表达水平显著低于对应癌旁组织(P<0.05);mi R-106a低表达与肿瘤淋巴结转移及高TNM分期(Ⅲ+Ⅳ期)显著相关(P<0.05);在前列腺癌组织中mi R-106a与Mcl-1及MMP2的表达呈显著负相关关系(P<0.05)。在前列腺癌LNCap细胞中过表达mi R-106a后可显著下调细胞内Mcl-1和MMP2 m RNA及蛋白的表达水平。结论:mi R-106a在前列腺癌组织中表达下调并与肿瘤恶性临床病理特征有关,mi R-106a可能通过下调Mcl-1及MMP2的表达来发挥抗肿瘤作用。Objective：To investigate the expression and clinical significance of micro RNA-106 a in human prostatic carcinoma（PC）. Methods： q RT-PCR was used to detect the relative expression of mi R-106 a in 43 paired PC and matched tumor adjacent tissues. The relationship between the mi R-106 a expression and clinical features were used Pearson chi-square test. The expressions of Mcl-1 and MMP2 were measured by immunohistochemistry（ IHC）,Spearman correlation analysis was used to analyze the relationship between mi R-106 a and Mcl-1 and MMP2. We then transfected the mi R-106 a mimics into LNCap cells,then the q RT-PCR and Western blot was used to detect the expression of Mcl-1 and MMP2,which were considered as the potential targets of mi R-106 a. Results： The relative expressions of mi R-106 a was significantly down-regulated in PC tissues compared with those of the matched tumor-adjacent tissues（ P 〈0. 05）. Low expression of mi R-106 a was significantly associated with tumor lymphatic metastasis and advanced TNM stage（Ⅲ ＋ Ⅳ）. The expressions of Mcl-1 and MMP2 were negative associated with mi R-106 a expression（P 〈0. 05）. Both the Mcl-1 and MMP2 m RNA and protein expression in LNCap cells were down-regulated after transfection. Conclusion： Low expression of mi R-106 a is related to the malignant clinicopathological features in PC tissues,and mi R-106 a may be through down-regulating Mcl-1 and MMP2 to suppress the progression of PC.

关 键 词：MicroRNA-106a 前列腺癌 MCL-1 MMP2 

分 类 号：R737.25[医药卫生—肿瘤]