兔肝VX2瘤血供程度对HIFU作用效果影响的初步研究  

Preliminary study on the relationship between the blood supply of rabbit liver VX2 tumor models and the HIFU

作　　者：周鹏[1] 贡雪灏[1] 周平[2] 何炜[2] 

机构地区：[1]深圳大学附属第一医院(深圳市第二人民医院)超声科,深圳518035 [2]中南大学湘雅三医院

出　　处：《中国医师杂志》2015年第7期1028-1031,共4页Journal of Chinese Physician

摘　　要：目的 探讨兔肝VX2瘤实质期血供程度对高能聚焦超声（HIFU）后肿瘤靶点处温度及凝固性坏死体积（V）大小的影响,为进一步精确控制HIFU剂量、提高治疗效率提供实验依据.方法 制作瘤兔24只并分成：10、15、20 d、无血供组（10 d成模后处死）4组,在对应时间进行相同参数HIFU辐照,对靶点实时测温,做出时间-温度曲线（TTC）,测量V,对坏死周边残存肿瘤组织及正常肝组织进行微血管密度（MVD）计数.结果 （1）肿瘤MVD：10 d组＞15 d组＞20 d组（P＜0.05）.（2）峰值温度（Tmax）、峰值时间（T1）、温升斜率（k1）：10 d组、15 d组、20 d组之间比较差异无统计学意义（P＞0.05）,此三组与无血供组比较差异有统计学意义（P＜0.05）.（3）温降斜率（k2）：10 d组＞15d组＞20 d组＞无血供组（P＜0.05）;温降时间（T2）：10 d组＜15 d组＜20 d组＜无血供组（P＜0.05）;（4）V：10 d组＜15 d组＜20 d组＜无血供组（P＜0.05）.结论 肿瘤血供程度对HIFU“温升”过程无显著影响,而对“冷却”过程有显著的影响.肿瘤血供越丰富,辐照后散热越快,组织越易恢复到正常温度,形成V越小.Objectives To explore the effects of the blood supply extent on the temperature of target site of tumor and volume of coagulation necrosis （V）,and to provide experimental evidence for further precise control of dosage of high intensity focused ultrasound （HIFU） and improve the efficiency of HIFU in the treatment.Methods Twenty-four rabbit liver VX2 tumor models were established and were divided into four groups：10 d group,15 d group,20 d group,no blood supply group （rabbits were put to death in the 10 d after the models were established）.The same irradiation parameters of HIFU were used to irradiate the hepatic tumor tissue of every group,the real-time temperature of target site of tumor were measured,the software from temperature controller was used to plot the time-temperature curve （TTC）,V was measured after HIFU.Residual tumor tissue was resected for pathological observation and microvascular density （MVD） determination.Results （1） Histopathological analysis showed that the extent of a tumor＆#39;s blood supply followed the order 10 d group 〉 15 d group 〉 20 d group （P 〈 0.01）.（2）Tmax,T1,k1：betweengroups had no statistical difference among 10 d group,15 d group,20 d group （P 〉0.05）,T T1,and k1 between three groups with no blood supply group had statistical difference （P 〈 0.05）.（3） k2：10 d group 〉 15d group 〉 20d group 〉 no blood supply group;T2：10d group 〈 15d group 〈 20d group 〈 no blood supply group;（4）V：10 d group 〈 15 d group 〈 20 d group 〈 no blood supply group.Conclusions The extent of a tumor＆#39;s blood supply had a significant effect on the temperature-decrease phase but not on the temperature-increase phase during HIFU treatment.The more abundant blood supply of the tumor was,the faster heat abstraction after HIFU was;and the easier the tissue return to normal temperature,the smaller the volume of coagulation necrosis tissue were.

关 键 词：肝肿瘤 实验性/血液供给/治疗 超声 高强聚焦 经肠 疾病模型 动物 

分 类 号：R735.7[医药卫生—肿瘤]