脑发育期缺碘对大鼠学习记忆功能的影响  

Influence of iodine deficiency in brain development on rat′s learning and memory functions

作　　者：李华娇[1] 

机构地区：[1]韶关学院医学院生理学教研室,广东韶关512026

出　　处：《中国当代医药》2015年第20期8-11,共4页China Modern Medicine

摘　　要：目的 探讨脑发育期缺碘对大鼠学习记忆功能的影响。方法 大鼠饮用含质量浓度为0.3 g/L甲硫咪唑的自来水诱发脑发育不同时期缺碘大鼠模型,E1组为胚胎期缺碘模型鼠,P1组为出生后1-50 d缺碘模型鼠,P30组为出生后30-50 d缺碘模型鼠,N组为正常对照鼠。观察其子代大鼠的行为学改变,利用放免法测定大鼠血清游离三碘甲腺原氨酸（FT3）和四碘甲腺原氨酸（FT4）水平,采用HE染色及TUNEL法观察大鼠的脑组织形态学改变。结果 P30组的血清游离FT3和FT4水平显著低于N组,差异有统计学意义（P〈0.05）。“Y”迷宫实验结果显示,P1和P30组的正确反应率显著低于N组,差异有统计学意义（P〈0.05）。P1组的达标所需天数显著多于N组,差异有统计学意义（P〈0.05）。P1组的记忆保持率显著低于N组,差异有统计学意义（P〈0.05）。P1组的正确反应率、记忆保持率显著低于E1组,达标所需天数显著多于E1组,差异有统计学意义（P〈0.05）。P1组的正确反应率显著低于P30组,达标所需天数显著多于P30组,差异有统计学意义（P〈0.05）。P1组和P30组的皮质细胞凋亡数显著多于N组,差异有统计学意义（P〈0.05）。P1组的皮质、海马细胞凋亡数显著多于E1组和P30组,差异有统计学意义（P〈0.05）。P1组的小脑细胞凋亡数显著多于E1组,差异有统计学意义（P〈0.05）。结论 脑发育时期缺碘能够导致大鼠学习记忆功能发育障碍,其发生与脑组织细胞凋亡增多有关。Objective To explore the influence of iodine deficiency in brain development on rat＇s learning and memory functions. Methods Iodine deficiency models of rats in different periods of brain development were induced by drinking tap water containing 0.3 g/L thiamazole.Iodine deficiency of model rat in embryonic period was classified into E1 group, iodine deficiency of model rats 1 to 50 d and 30 to 50 d after birth were named as P1 group and P30 group respectively,and normal control group was set as N group.Change of behavioristics in offspring rats was observed.Serum free triiodothryonine （FT3） and free thyroxine or tetraiodothyronine （FT4） was determined by radioimmunoassay respectively. Morphological change of rat＇s brain tissue was observed by HE staining and TUNEL. Results The level of serum FT3 and FT4 in P30 group was lower than that in E1 group and P1 group,with significant difference （P〈0.05）.In the ＂Y＂ maze experiments,the rate of correct response in P1 group and P30 group N group was lower than that in N group,with significant difference （P〈0.05）.The days of reachting the standards in P1 group was more than that in N group,with significant difference（P〈0.05）.The rate of memory retention in P1 group was lower than that in N group,with significant difference （P〈0.05）.The rate of correct response and memory retention in P1 group was lower than that in E1 group,with significant difference（P〈0.05）.The days of reaehting the standards in P1 group was more than that in E1 group,with significant difference （P〈0.05）.The rate of correct response in P1 group was lower than that in P30 group,with significant difference（P〈0.05）.The days of reachting the standards in P1 group was more than that in P30 group,with significant difference （P〈0.05）.The apoptosis number of cerebral cortex cell in P1 group and P30 group was more than that in N group,with significant difference （P〈0.05）.The apoptosis number of cerebral cortex and hippocampus cell was more than

关 键 词：脑发育 缺碘 大鼠 学习记忆功能 

分 类 号：R-332[医药卫生]