NDV、KLT及其联用时小鼠H_(22)肝癌瘤组织的病理变化与TNF-α表达的研究  

Effects of NDV,KLT and their combination on pathological changes of tumor tissue and expression of TNF-αof mice with H_(22) liver cancer

作　　者：周琳[1] 张琪[1] 梁帅[1] 余关林 赵善廷[1] 许信刚[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2015年第7期15-23,共9页Journal of Northwest A&F University(Natural Science Edition)

基　　金：西北农林科技大学基本科研业务费项目(Z109021427);西北农林科技大学博士科研启动基金项目(Z109021116)

摘　　要：【目的】研究新城疫病毒(New Castle Disease Virus,NDV)、康莱特注射液(Kanglaite Injection,KLT)及NDV+KLT联用治疗肝癌肿瘤过程中,癌组织的病理变化以及肿瘤坏死因子-α(Tumor necrosis factor-alpha,TNF-α)在肿瘤组织中的表达情况,探究TNF-α在抗肿瘤过程中的作用,为应用生物素治疗肿瘤提供一定的理论依据。【方法】建立小鼠H22肝癌肿瘤模型,将96只荷瘤小鼠随机均分成生理盐水对照组(0.8mL/只)、NDV治疗组(0.8mL/只)、KLT治疗组(0.5mL/只)及NDV+KLT联合治疗组共4组,分别在治疗后的4,8,12和16d取小鼠肿瘤组织制作2套石蜡切片,一套进行HE染色检测病理变化;另一套进行免疫组织化学SP观察TNF-α的表达情况。【结果】HE染色发现,与对照组相比,NDV组和KLT组都有明显的肿瘤生长抑制及肿瘤细胞坏死现象,特别是NDV+KLT联合治疗组的治疗效果显著;免疫组化研究结果表明,不同的治疗组TNF-α分布差异显著,其中NDV+KLT联合治疗组的阳性反应最强,其次是KLT治疗组。【结论】NDV和KLT的抗肿瘤作用与TNF-α细胞因子表达有一定的相关性,其中NDV+KLT联合治疗的抗肿瘤效果最好。This study used New Castle Disease Virus （NDV）,Kanglaite Injection （KLT） and their combination to treat liver cancer. The pathological changes of tumor tissue and expression of tumor necrosis factor-alpha （TNF-α） were analyzed to understand the function of TNF-α in cancer treatment. [Method] Mice H22 tumor model was established with 96 tumor-bearing mice randomly divided into four groups,NS control group （0. 8 mL）,NDV treatment group （0. 8 mL）,KLT treatment group （0. 5 mL） and NDV＋KLT treatment group. Tumor tissue was obtained from every group in the 4,8,12 and 16 d respectively. Paraffin sections were made to observe pathological changes using hematoxylin-eosin （HE） staining and expression of TNF-α using immunohistochemistry SP method. [Result] HE staining showed that, compared with NS control group, tumor growth inhibition and tumor cells necrosis appeared, especially NDV＋KLT treatment group had significant treatment effect. Compared with NS control group, patho-logical changes of tumor tissue and distribution of TNF-α were signific groups, especially in NDV ＋ KLT group. NDV＋ KLT group also had the antly different in all treatment strongest positive reaction, followed by KLT treatment group. [Conclusion] Anti-tumor effect of NDV and KLT was related to expres- sion of TNF-a and combination of NDV and KLT had the best effect.

关 键 词：TNF -α NDV KLT H22肝癌 

分 类 号：S852.3[农业科学—基础兽医学]