DEK表达下调通过抑制胃癌SGC-7901细胞中NF-κB信号途径诱导细胞凋亡  

Downregulation of DEK induces cell apoptosis via inhibition of NF-κB signaling pathway in gastric carcinoma SGC-7901 cells

作　　者：张彩凤[1] 董良鹏[2] 夏永华[3] 郭晓鹤[1] 张利利[1] 周慧聪[1] 张兰芳[1] 李贞娟[1] 韩宇[1] 

机构地区：[1]新乡医学院第一附属医院消化内科,河南卫辉453100 [2]新乡医学院第一附属医院普通外科,河南卫辉453100 [3]新乡医学院第一附属医院皮肤科,河南卫辉453100

出　　处：《中国病理生理杂志》2015年第7期1197-1202,共6页Chinese Journal of Pathophysiology

基　　金：河南省教育厅科技攻关项目(No.2009B320008);河南省卫生厅科技攻关项目(No.200804055);新乡医学院科研培育基金(No.2013QN128)

摘　　要：目的:研究DEK表达下调对胃癌SGC-7901细胞凋亡的影响,并分析其与NF-κB信号途径及凋亡相关蛋白表达的关系。方法:将DEK siRNA和对照siRNA转染胃癌SGC-7901细胞,并将SGC-7901细胞分为未处理组、对照siRNA组及DEK siRNA组。采用实时荧光定量PCR和Western blot检测3组胃癌SGC-7901细胞中DEK mRNA和蛋白的表达;流式细胞术检测3组SGC-7901细胞凋亡的变化;Caspase-Glo-3/9试剂盒检测3组SGC-7901细胞中caspase-3和caspase-9的活性;Western blot技术检测3组SGC-7901细胞中NF-κB信号途径关键调控蛋白p65及凋亡相关蛋白Bcl-2和Bax的表达。结果:与未处理组和对照siRNA组相比,DEK siRNA组SGC-7901细胞中DEK的mRNA和蛋白表达水平显著下调(P<0.05)。DEK siRNA组SGC-7901细胞的早期凋亡率和总凋亡率均显著高于未处理组和对照siRNA组(P<0.05)。最为显著的是,DEK siRNA转染细胞后,p65和Bcl-2蛋白表达下调,Bax蛋白表达上调,并伴随caspase-3和caspase-9活性上升。结论:DEK表达下调介导的SGC-7901细胞凋亡可能与NF-κB信号途径密切相关。AIM： To investigate the effect of DEK downregulation on the apoptosis of gastric carcinoma SGC-7901 cells,and to explore its associations with NF-κB signaling pathway and apoptosis related proteins. METHODS：SGC-7901 cells with different treatments were divided into 3 groups including untreated group,control siRNA group and DEK siRNA group. The expression of DEK at mRNA and protein levels in the SGC-7901 cells was detected by real-time PCR and Western blot. The cell apoptosis was examined by flow cytometry. Furthermore,the activities of caspase-3 and caspase-9 in the SGC-7901 cells were investigated by Caspase-Glo-3 /9 kit. Finally,the expression of key regulatory protein p65 of NF-κB signaling pathway and apoptosis-related proteins Bcl-2 and Bax in the SGC-7901 cells was investigated by Western blot. RESULTS： Compared with untreated group and control siRNA group,the expression of DEK at mRNA and protein levels was significantly downregulated in DEK siRNA group（ P 0. 05）. In addition,the ratios of early phase apoptosis and total apoptosis in DEK siRNA group were markedly higher than those in untreated group and control siRNA group（ P 0. 05）. Most notably,the decrease in p65 and Bcl-2 proteins,increase in Bax protein and the increases of caspase-3 and caspase-9 activities were observed in DEK siRNA group. CONCLUSION： Downregulation of DEK mediates cell apoptosis of gastric carcinoma may be tightly associated with NF-κB signaling pathway.

关 键 词：胃癌 DEK 细胞凋亡 NF-κB信号途径 

分 类 号：R730.23[医药卫生—肿瘤] R735.2[医药卫生—临床医学]