Over-expression of recombinant proteins with N-terminal His-tag via subcellular uneven distribution in Escherichia coil  

作　　者：Won-Ji Park Sung-Hwan You Hyoung-An Choi Yeon-Jin Chu Geun-Joong Kim 

机构地区：[1]Department of Biological Sciences, College of Natural Sciences, Chonnam National University, ISwangju 500-757,Republic of Korea

出　　处：《Acta Biochimica et Biophysica Sinica》2015年第7期488-495,共8页生物化学与生物物理学报（英文版）

摘　　要：Specific tags with defined amino acid residues are widely used to purify or probe target proteins. Interestingly, the tagging system occasionally results in an increase of the recombinant protein expression in vivo. Here, we systematically examined this phenomenon using a poly-histidine （His）-tag fused to N- or C-terminal region of green, red, and blue fluorescent proteins by quantifica- tion and uneven distribution in cytoplasm of Escherichia coil This effect was further supported by the distinct over-expression of several unrelated proteins, such as esterase, neopullulanase, and chloramphenicol acetyltransferase, tagging with the same tag. These results suggest that a poly- His-tag placed at N-terminal region can induce over-expression of recombinant protein via subcellu- lar uneven distribution in vivo.Specific tags with defined amino acid residues are widely used to purify or probe target proteins. Interestingly, the tagging system occasionally results in an increase of the recombinant protein expression in vivo. Here, we systematically examined this phenomenon using a poly-histidine （His）-tag fused to N- or C-terminal region of green, red, and blue fluorescent proteins by quantifica- tion and uneven distribution in cytoplasm of Escherichia coil This effect was further supported by the distinct over-expression of several unrelated proteins, such as esterase, neopullulanase, and chloramphenicol acetyltransferase, tagging with the same tag. These results suggest that a poly- His-tag placed at N-terminal region can induce over-expression of recombinant protein via subcellu- lar uneven distribution in vivo.

关 键 词：HIS-TAG OVER-EXPRESSION uneven distribution IMMUNOFLUORESCENCE 

分 类 号：Q786[生物学—分子生物学] Q78