趋化因子CCL18对前列腺癌细胞迁移力、侵袭力和细胞增殖影响的研究  

Effects of CCL18 on the tumor invasion, migration and proliferation abilities of human cancer cells

作　　者：罗杰鑫[1] 叶永康[1] 陈果[2] 吴永定 米其武[1] 梁桂锋 叶剑恒 李博伟[2] 钟惟德[2] 

机构地区：[1]东莞市人民医院泌尿外科,广东省523059 [2]广州医科大学附属广州市第一人民医院泌尿外科 广东省临床分子医学及分子诊断重点实验室,510180

出　　处：《中华临床医师杂志（电子版）》2015年第13期76-78,共3页Chinese Journal of Clinicians(Electronic Edition)

基　　金：国家自然科学基金(81170699);广州市科技计划重点项目(23150711);广东省泌尿外科重点实验室项目(060801016)

摘　　要：目的研究趋化因子CCL18对前列腺肿瘤细胞侵袭力、迁移力和细胞增殖的影响。方法通过培养前列腺肿瘤细胞LNCa P、DU145,并加入趋化因子蛋白CCL18,利用Transwell实验、Wound Healing实验和CCK8试剂盒来分别检测CCL18对前列腺肿瘤细胞侵袭力、迁移力及对细胞增殖的影响。结果经过趋化因子蛋白CCL18处理的前列腺肿瘤细胞,在Transwell实验中,肿瘤细胞跨膜细胞数显著增加(LNCa P:对照vs.CCL18=202.0±18.5 vs.279.7±27.6;DU145:对照vs.CCL18=60.3±6.5 vs.91.0±9.5);Wound Healing实验中,肿瘤细胞发生迁移的数量明显增加(LNCa P:对照vs.CCL18=127.3±14.3 vs.214.4±30.9;DU145:对照vs.CCL18=68.6±15.8 vs.129.4±12.0);细胞生长实验结果揭示肿瘤细胞增殖速度也显著加快。结论趋化因子蛋白CCL18能促进前列腺肿瘤细胞LNCa P、DU145的侵袭力、转移力,并加快前列腺肿瘤细胞的生长速度。ObjectiveTo study the effects of CCL18 on tumor invasion, migration and proliferation of prostate cancer cells.MethodsIn this study, LNCaP and DU145 cell lines were treated by CCL18, the tumor invasion, migration and proliferation abilities were detected by transwell assay, migration assay and CCK8 assay, respectively.ResultsThe results showed that CCL18 promoted the abilities of tumor invasion （LNCaP： Ctrlvs. CCL18=202.0±18.5vs. 279.7±27.6; DU145： Ctrlvs. CCL18=60.3±6.5vs. 91.0±9.5） and migration （LNCaP：Ctrlvs. CCL18=127.3±14.3vs. 214.4±30.9; DU145： Ctrlvs. CCL18=68.6±15.8vs. 129.4±12.0）, and display a higher proliferation rate in prostate cancer.ConclusionCCL18 could significantly enhance the tumor invasion, migration and proliferation abilities of both prostate cancer cell lines LNCaP and DU145.

关 键 词：细胞增殖 CCL18 前列腺癌细胞 侵袭力 迁移力 

分 类 号：R737.25[医药卫生—肿瘤]