柱前衍生化HPLC法测定不同基原珍珠粉及贝壳粉中7种氨基酸的含量  

Determination of Amino Acids in Pearl Powder and Conch Powder from Different Origins by HPLC with Pre-column Derivation

作　　者：陈佳[1] 魏锋[1] 程显隆[1] 刘薇[1] 马双成[1] 

机构地区：[1]中国食品药品检定研究院,北京100050

出　　处：《中药材》2015年第4期693-696,共4页Journal of Chinese Medicinal Materials

基　　金：国家十二五"重大新药创制"专项(2014ZX09304307-002);国家自然科学基金青年项目(81202909);中国食品药品检定研究院中青年研究发展基金(2014A7)

摘　　要：目的：建立柱前衍生化HPLC检测不同基原珍珠粉及贝壳粉中7种主要氨基酸的方法。方法：以异硫氰酸苯酯为柱前衍生化试剂,采用C18色谱柱,流动相A：0.1mol/L醋酸钠溶液（用醋酸调pH值至6.5）,流动相B：乙腈,梯度洗脱,检测波长254nm。结果：氨基酸衍生溶液在24h内保持稳定,7种氨基酸线性相关系数均大于0.9995,加样回收率在93.16%~103.31%之间。结论：本方法分离效果好,精密度和重复性良好,可用于珍珠粉及贝壳粉中7种水解氨基酸含量的测定。Objective： To establish an analysis method for determination of amino acids in pearl powder and conch powder from different origins by HPLC with pre-column derivation. Methods： Hydrolytic amino acids were extracted by acid hydrolysis method,then derivatized with phenyl isothiocyanate（ PITC）. And the samples were analyzed on a C18 column with gradient elution of 0. 1 mol / L sodium acetate solution（ adjusted to p H 6. 5）（ A） and acetonitrile（ B） at the flow rate of 1. 0 m L / min. The detected wavelength was 254 nm. Results： Amino acids derivative solution remained stable in 24 hours. The response was linear for seven amino acids with a correlation coefficient r 0. 9995. The average recoveries were 93. 16% ~ 103. 31%. Conclusion： The method is sensitive with good accuracy and repeatability,which is useful for the determination of amino acids in pearl powder and conch powder from different origins.

关 键 词：珍珠粉 贝壳粉 异硫氰酸苯酯 柱前衍生化 氨基酸 HPLC 

分 类 号：R282.74[医药卫生—中药学]