两个产地石菖蒲挥发油质量比较研究  

Comparative Study on Quality of Volatile Oil of Acori Tatarinowii Rhizoma from Two Habitats

作　　者：陈小露[1] 袁德俊[1,2] 吴雪茹[2] 吴启端[1] 

机构地区：[1]广州中医药大学第一附属医院,广东广州510405 [2]广州中医药大学附属骨伤科医院,广东广州510240

出　　处：《中药材》2015年第4期770-773,共4页Journal of Chinese Medicinal Materials

基　　金：广州中医药大学中医药科研创新基金项目(10CX064)

摘　　要：目的:比较广西和四川两个产地的石菖蒲挥发油质量。方法:采用水蒸气蒸馏法提取挥发油,GC-MS技术对挥发油化学成分进行分析鉴定,峰面积归一化法计算各成分的相对百分含量。并以主要成分甲基丁香酚、顺式甲基异丁香酚、γ-细辛醚、β-细辛醚、α-细辛醚的相对百分含量之和为指标进行聚类分析。结果:广西和四川两产地各10批石菖蒲挥发油的平均提取率为1.61%、1.72%;5个主要成分的相对百分含量合计分别为78.19%和88.84%;经t检验,两者无统计学差异。广西产10批石菖蒲样品聚类可分为4小类,四川产10批石菖蒲样品聚类可分为5小类,而两产地均数与两产地20批样品进行聚类均在同一小类里;广西产10批石菖蒲样品的相关系数中有8批在0.9以上,2批在0.9以下,其中1批的相关系数只有0.466,四川产10批石菖蒲样品的相关系数中有7批在0.9以上,3批在0.9以下,其中1批的相关系数只有0.069,而两产地均数之间的相关系数为0.996。结论:不同批次的石菖蒲挥发油质量差异较大,但与两产地所属省份没有相关性。Objective： To compare the quality of volatile oil of Acori Tatarinowii Rhizoma from Guangxi and Sichuan. Methods： The volatile oil was extracted from Acori Tatarinowii Rhizoma by using steam distillation method,and analyzed by GC-MS. Peak area normalization method was used for calculating the relative percentage contents of chemical constituents,and hierarchical cluster analysis was used for classifying the 20 batches of samples by their relative contents of the main components that were methyleugenol,cis-methylisoeugenol,γ-asarone,β-asarone and α-asarone. Results： The average extraction rate of 10 batches of volatile oil in Acori Tatarinowii Rhizoma from Guangxi was 1. 61%,and 10 batches of samples from Sichuan was 1. 72%. The relative percentage contents of five main components totaled 78. 19% and 88. 84%,respectively. By t-test,there was no statistical difference between samples from Guangxi and Sichuan. In the hierarchical cluster analysis,10 batches of samples from Guangxi and 10 batches of samples from Sichuan could respectively be classified into four clusters subcategories and five clusters subcategories,while the mean of samples of Guangxi and the mean of samples of Sichuan respectively analyzed with 20 batches of the two habitats that all were classified in the same clusters subcategories. The results of similarity showed that the correlation coefficients of 8 batches in 10 batches of samples from Guangxi were over 0. 9,while 1 batch was only 0. 466. The correlation coefficients of 7 batches in 10 batches of samples from Sichuan had were over 0. 9,while1 batch was only 0. 069. The correlation coefficients between the mean of samples of Guangxi and the mean of samples of Sichuan was0. 996. Conclusion： Quality of the different batches of volatile oil from Acori Tatarinowii Rhizoma have significant differences,but it has no obvious correlation with the habitats.

关 键 词：石菖蒲 挥发油 化学成分 气相色谱-质谱联用技术 

分 类 号：R284.2[医药卫生—中药学] R286[医药卫生—中医学]