Erode-Dilate结合球形三维分析法在Micro-CT评估牙槽骨量中的应用  

Quantification of alveolar bone with Erode-Dilate method combined with the sphere module in Micro-CT analysis

作　　者：刘雪飞[1] 张旭[1] 林江[1] 

机构地区：[1]哈尔滨医科大学附属第四医院口腔科,黑龙江哈尔滨150001

出　　处：《牙体牙髓牙周病学杂志》2015年第7期422-426,438,共6页Chinese Journal of Conservative Dentistry

基　　金：黑龙江省留学归国科学基金项目(LC201435)

摘　　要：目的:用对偶运算对实验性牙周炎小鼠牙槽骨的Micro-CT图像进行腐蚀-膨胀(Erode-Dilate)处理,并结合Med CAD模块中的球形三维分析法定量分析其牙槽骨余留量。方法:将20只C57小鼠随机分为2组(n=10),实验组小鼠采用丝线结扎(2周)法建立上颌第二磨牙牙周炎模型,对照组不作处理。建模后,分别对各组小鼠的上颌骨进行Micro-CT扫描,运用常规线性二维分析法以及Erode-Dilate结合Med CAD模块中的球形三维分析法对各小鼠的余留牙槽骨骨量进行计算评估;分析两种方法结果的可靠性和可重复性,并同时比较两种方法所需的操作时间。结果:采用二维线性分析时,实验组的牙槽骨余留量与对照组有显著性差别(P<0.05)。而采用Erode-Dilate结合Med CAD模块中的球形三维分析法时,不仅骨量有显著差异,而且骨密度也有显著差异(P<0.05);统计学分析结果显示,该分析法的可靠性和可重复性均相对较高,所需时间也明显少于二维分析法(P<0.05)。结论:Erode-Dilate结合Med CAD模块中的球形三维分析法可对小鼠MicroCT扫描图像的牙槽骨余留骨量进行准确、快速的定量分析,并具有良好的灵敏性和可重复性。AIM： To evaluate the remaining alveolar bone around molars quantitatively in mice with the mathematical morphology method of Erode-Dilate and sphere module for the Micro-Computed Tomography（ Micro-CT）images. METHODS： The second maxillary molars were ligated with silk in maxilla bone of 10 C57 mice for 2 weeks and 10 mice with out ligation were served as the control. The maxilla bones were scanned with Micro-CT. And then the remaining alveolar bone was measured with two-dimensional linear method and Erode-Dilate and sphere module threedimensional method respectively. The difference between interexaminer and intraexaminer,and the running time were analyzed statistically. RESULTS： The two- dimensional linear method showed that the remaining alveolar bone was statistically different between control and experimental groups. But the three-dimensional method revealed the statistically difference not only in bone volume but also in density（ P〈0. 05）. The reliability and repeatability of Erode-Dilate and sphere module three- dimensional method was higher and more efficient than two- dimensional linear method（P〈0. 05）. CONCLUSION： Silk ligature can induce significant alveolar bone resorption in mice in 2 weeks. Erode-Dilate and sphere module three-dimension method can be used quickly and accurately to evaluate the remaining alveolar bone with Micro-CT images in mice.

关 键 词：微计算机断层扫描技术 牙槽骨 牙周炎 数学形态学 腐蚀-膨胀 

分 类 号：R780.4[医药卫生—口腔医学]