84例子宫内膜癌中Foxp3的表达及临床意义  

Expression and clinical significance of Foxp3 in 84 cases of human endometrial carcinoma

作　　者：张雅丽[1] 侯安丽[1] 王杏茶[1] 许倩[2] 陈龙[2] 岳志玲 

机构地区：[1]承德医学院附属医院妇科,承德067000 [2]承德医学院基础医学研究所,承德067000

出　　处：《临床与实验病理学杂志》2015年第7期748-751,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的探讨调节性T细胞的表面标志物Foxp3 mRNA及蛋白在子宫内膜癌组织和正常子宫内膜组织中的表达及其临床意义。方法应用免疫组化SP法及实时荧光定量PCR法检测84例子宫内膜癌组织和40例正常子宫内膜组织中Foxp3 mRNA及蛋白的表达,分析Foxp3在子宫内膜癌组织中的表达与肿瘤组织分化程度和FIGO分期的关系。结果子宫内膜癌组织Foxp3表达明显高于正常子宫内膜组织;Foxp3表达与子宫内膜癌FIGO分期有关,Ⅲ+Ⅳ期子宫内膜癌患者Foxp3表达高于Ⅰ+Ⅱ期患者(P<0.05)。Foxp3表达与分化程度的关系在上述两种检测方法中得出不同结论,免疫组化检测提示Foxp3蛋白表达与组织分化等级间存在相关性(rs=0.72,P<0.01),中+低分化组Foxp3+细胞数明显高于高分化组(P<0.01)。而实时荧光定量PCR法检测提示Foxp3 mRNA表达与肿瘤组织学分级无相关性(rs=0.01,P=0.35)。结论 Foxp3在子宫内膜癌患者癌灶中高表达,免疫组化检测的蛋白表达与实时荧光定量PCR检测结果不完全一致,Foxp3可能参与了子宫内膜癌的发生、发展。Purpose To investigate the expression and clinical significance of Foxp3 （ cell surface marker of regulatroy T） mRNA and its protein in endometrial carcinomas and normal endometrial tissues. Methods Real-time fluorescence quantitative PCR and immuno-histochemical SP methods were used to detect the expressions of mRNA and protein in tumor tissue of 84 cases of endometrial carcino-mas and 40 cases of normal endometrial tissue, then to analyze the relationship between Foxp3 gene and clinical pathological character-istics of endometrial carcinoma specimens, such as differentiation, FIGO stage. Results Foxp3 mRNA and it′s protein expression of endometrial carcinomas were significantly higher than that of normal endometrial tissues. There were significantly relationships between Foxp3 mRNA expression and FIGO stage of endometrial cancer, Foxp3 mRNA expressions of III＋IV stage was higher than that ofⅠ＋Ⅱ stage endometrial carcinoma （P〈0. 05）. But the relationship between Foxp3 expression and differentiation degree reached differ-ent conclusions in the two detection methods. By immunohistochemistry the expression of Foxp3 protein was correlation with histological differentiation grade （rs =0. 72, P 〈0. 01）. In poorly differentiated endometrial carcinoma Foxp3 ＋ cell number was significantly higher than that in well differentiated endometrial carcinoma. By detection of real-time fluorescence quantitative PCR method, Foxp3 mRNA expression was not correlated with tumor grade （rs =0. 01, P=0. 35）. Conclusion Foxp3 in endometrial carcinomas are high expressions. Immunohistochemical method has more clinical value than real-time fluorescence quantitative PCR test results. Foxp3 may be involved in the regulation of the development of endometrial cancers.

关 键 词：子宫肿瘤 子宫内膜癌 FOXP3 TREG细胞 

分 类 号：R737.33[医药卫生—肿瘤]