慢性刺激大鼠海马微管相关蛋白-2的表达及氟西汀联合丰富环境的作用  

Expression of microtubule associated protein-2 in hippocampus and effect of fluoxetine combine with enrich environment in chronic unpredictable stress rats

作　　者：刘聪[1] 王长虹[1] 闫福林[2] 韩金红[3] 谷景阳 翟飞[1] 单筱雯 

机构地区：[1]新乡医学院第二附属医院临床精神医学教研室,453002 [2]新乡医学院药学院药物化学与药剂学教研室 [3]新乡医学院基础医学院组织与胚胎学教研室

出　　处：《中华精神科杂志》2015年第4期239-245,共7页Chinese Journal of Psychiatry

基　　金：河南省基础与前沿技术研究计划项目（142300410025）;河南省科技攻关计划项目（112102310211）;新乡医学院科研项目培育基金（2013ZD117）;新乡医学院高层次人才科研资助项目（08BSKYQD-004）;新乡医学院研究生科研创新支持计划资助项目（YJSCX201243Y）

摘　　要：目的 探讨慢性不可预见性刺激（chronic unpredictable stress,CUS）大鼠海马微管相关蛋白-2（microtubule associated protein-2,MAP-2）表达水平的变化,氟西汀联合丰富环境对CUS大鼠的作用.方法 将30只成年雄性Sprague-Dawle（SD）大鼠采用随机数字表法分为CUS组、正常组、CUS＋氟西汀组、CUS＋丰富环境组、CUS＋氟西汀＋丰富环境组,每组6只.CUS组均孤养并接受6周CUS,正常组标准环境每笼3只群养6周,剩余3组接受6周CUS并于第3周末至第6周末分别氟西汀、丰富环境、氟西汀＋丰富环境干预,CUS前后及干预后以糖水消耗实验、体重测量、旷场实验对大鼠行为进行评估,以免疫组织化学法测定各组大鼠海马MAP-2表达水平.结果 （1）CUS前各组大鼠行为评估组间两两比较,糖水消耗、体重、水平运动距离、直立次数、粪便粒数差异均无统计学意义.（2）CUS后CUS组、CUS＋氟西汀组、CUS＋丰富环境组、CUS＋氟西汀＋丰富环境组较正常组糖水消耗少、体重增加少、水平运动距离小（均P＜0.05）.（3）干预后正常组、CUS＋氟西汀组、CUS＋丰富环境组、CUS＋氟西汀＋丰富环境组较CUS组糖水消耗多、体重增加多、水平运动距离大（均P＜0.05）;正常组[（84±3）g,（6 687±664） cm]较CUS＋氟西汀组[（75±4）g,（5 859±624） cm]、CUS＋丰富环境组[（77±8）g,（5 876±784） cm]体重增加多（P=0.005、0.029）、水平运动距离大（P=0.028、0.031）;CUS＋氟西汀＋丰富环境组[（6 657±430） cm]较CUS＋氟西汀组、CUS＋丰富环境组水平运动距离大（P=0.033、0.037）.（4）免疫组织化学显示,正常组（0.408±0.014、0.405±0.011、0.406±0.012）、CUS＋氟西汀组（0.403±0.011、0.403±0.011、0.403±0.012）、CUS＋丰富环境组（0.406±0.015、0.399±0.013、0.406±0.017）、CUS＋氟西汀＋丰富环境组（0.407±0.015、0.401±0.010、0.407±0.013）MAP-2在海马CA1、CA3和齿状回区Objective To discuss the change of the expression level of microtubule associated protein-2 （MAP-2） in hippocampus and the effect of fluoxetine combine with enrich environment in chronic unpredictable stress （CUS） rat.Methods Divided 30 male Sprague-Dawle （SD） rats into CUS group,normal group,CUS ＋ fluoxetine group,CUS ＋ enrich environment group,and CUS ＋ fluoxetine ＋ enrich environment group averagely and randomly according to random number table （n=6 each）.Rats in CUS group were all fed lonely and received CUS for 6 weeks.Rats in normal group were fed in groups （3 rats in every cage） in standard environment for 6 weeks.Rats in CUS＋fluoxetine group,CUS＋enrich environment group,and CUS ＋ fluoxetine ＋ enrich environment group received CUS for 6 weeks and had a lavage with fluoxetine,an intervention of enrich environment,a lavage with fluoxetine ＋ an intervention of enrich environment respectively during the last 3 weeks every day.Every rat went a behavioral assessment before and after CUS and after intervention.Behavioral assessment included sucrose water consumption test,weight measurement,and open field test （horizontal moving distance,number of vertical,number of faece）.Measured the level of MAP-2 expressed in hippocampus with immunohistochemistry at last.Results （1） Behavioral assessment before CUS：there was no significant difference between CUS group,normal group,CUS ＋ fluoxetine group,CUS ＋ enrich environment group and CUS ＋ fluoxetine ＋ enrich environment group in sucrose water consumption test,weight measurement,horizontal moving distance,number of vertical and number of faece.（2） Behavioral assessment after CUS：consumption of sucrose water,gain of body weight,distance of horizontal moving of rats in CUS group,CUS ＋ fluoxetine group,CUS ＋ enrich environment group,and CUS＋fluoxetine＋enrich environment group were all less than rats in normal group （all P〈0.05）.（3）Behavioral assessment after intervention：consumption of sucrose w

关 键 词：抑郁症 大鼠 海马 氟西汀 慢性不可预见性刺激 丰富环境 

分 类 号：R749.5[医药卫生—神经病学与精神病学]