鱼油对腹腔感染小鼠肠道潘氏细胞功能的影响  

Effect of fish oil on intestinal Paneth cells in mouse with abdominal infection

作　　者：田锋[1] 王新颖[1] 高学金[2] 万晓[1] 

机构地区：[1]南京大学医学院附属金陵医院解放军普通外科研究所,210002 [2]南方医科大学临床学院南京军区南京总医院普通外科

出　　处：《中华胃肠外科杂志》2015年第7期702-706,共5页Chinese Journal of Gastrointestinal Surgery

基　　金：国家自然科学基金资助项目（81470797）

摘　　要：目的探讨腹腔感染后肠道潘氏细胞功能的改变及鱼油的干预作用。方法将50只C57BL／6J小鼠随机分为5组，每组10只。腹腔感染组施行盲肠结扎穿孔手术；鱼油组在盲肠结扎穿孔术后给予鱼油灌胃；长链脂肪乳组在盲肠结扎穿孔术后给予大豆油脂肪乳；假手术组只进行腹腔麻醉和外科操作，不施行盲肠结扎穿孔：正常对照组不作任何处理。术后4d处死小鼠，观察体质量变化，采用免疫组织化学染色进行病理学观察，采用qRT—PCR检测小鼠末端回肠的溶菌酶、隐窝素4和分泌型磷脂酶A2mRNA表达，Western blot法检测溶菌酶的蛋白表达水平。结果与正常对照组比较，腹腔感染组小鼠体质量明显减轻（P〈0．01），溶菌酶、隐窝素4和分泌型磷脂酶A2mRNA表达水平明显降低（0．78±0．34比1．83±0．11；0．99±0．44比2．02±0．33；0．92±0．25比1．50±0．27；均P〈0．05），溶菌酶的蛋白表达水平也明显降低（0．31±0．06比0．45±0．05；P〈0．05），小肠绒毛倒伏、断裂明显。与腹腔感染组和长链脂肪乳组比较，鱼油组的溶菌酶mRNA和蛋白表达水平明显升高（1．23±0．27比0．78±0．34和0．62±0．23；0．38±0．07比0．31±0．06和0．32±0．06：均P〈0．05）．小肠绒毛倒伏和断裂现象减轻。结论腹腔感染状态下，肠道潘氏细胞功能明显受损，鱼油能改善这一损伤。Objective To investigate the effect of fish oil on intestinal Paneth cells in mouse with abdominal infection. Methods Fifty C57BL/6J mouse were randomly divided into five groups（n=10 each）： control group, sham group, infection group （cecal ligation and puncture,CLP）, fish oil group （0.4 g/kg fish oil, intragastric administration every day, FO） and long chain triglyceride group （0.4 g/kg soybean oil, intragastric administration every day, LCT）. The mouse were sacrificed and the terminal ileum was collected for lysozyme, cryptdin 4 and secreted phosphatidase A2（sPLA2） analysis at the fourth day after operation. The changes of mouse body weight and intestinal mucosa pathology were observed. Results The body weight, the mRNA levels of lysozyme, cryptdin 4 and sPLA2 and the protein level of lysozyme of Paneth cells in the infection group were reduced compared with the control group （0.78±0.34 vs. 1.83±0.11, 0.99±0.44 vs. 2.02±0.33, 0.92±0.25 vs. 1.50±0.27, 0.31±0.06 vs. 0.45 ±0.05, all P〈0.05）, meanwhile the intestinal villi collapse and breakage occurred obviously. Fish oil could up-regulate the mRNA and protein expression of lysozyme （ 1.23±0.27 vs. 0.78±0.34 and 0.62±0.23, 0.38±0.07 vs. 0.31±0.06 and 0.32±0.06, all P〈0.05） and alleviate the mueosa injury compared with the infection group and LCT group. Conelttsions The function of intestinal Paneth cells is damaged apparently after cecal ligation and puncture. Fish oil can relieve this injury.

关 键 词：腹腔感染 肠黏膜屏障 潘氏细胞 鱼油 脂肪酸 小鼠 

分 类 号：R459.3[医药卫生—治疗学]