聚乙烯亚胺纳米粒递送siRNA的抗肿瘤生长作用  

Effect of delivering siRNA with polyethylenimine nanoparticles on inhibiting tumor growth

作　　者：王海杰[1] 谭玉珍[1] 

机构地区：[1]复旦大学上海医学院人体解剖与组织胚胎学系,上海200032

出　　处：《解剖科学进展》2015年第4期401-404,共4页Progress of Anatomical Sciences

基　　金：国家自然科学基金资助项目(No.30570948);高等学校博士点专项科研基金项目(No.20130071110080)

摘　　要：聚乙烯亚胺(polyethylenimine,PEI)是一种阳离子聚合物,能够结合并压缩siRNA,形成稳定的PEI/siRNA纳米复合物。PEI纳米复合物通过静电作用与细胞膜结合,以胞吞方式进入细胞内。在质子化作用下纳米复合物逃离溶酶体,使携带的siRNA免受溶酶体酶降解,从而靶向作用于目的mRNA。对PEI纳米粒进行表面修饰后,可显著降低其细胞毒性和提高siRNA递送效率。因此,这种PEI纳米粒递送siRNA先进技术的有效利用对于肿瘤治疗有着广阔的应用前景。Polyethylenimine（PEI）, a cationic polymer, can bind and compress siRNA to form stable PEI/siRNA nanocomplexes. The nanocomplexes bind cell membrane by electrostatic interaction and then enter the cells by phagocytosis. With protonation mechanism, PEI nanoparticles escape from lysosomes so that degradation of the carried siRNA is avoided. Therefore, siRNA delivered with PEI nanoparticles targets the aimed mRNA. After modification of the surface of PEI nanoparticles, cytotoxicity of PEI decreases significantly, efficiency of siRNA delivery increases. Application of the technique of siRNA delivery with PEI nanoparticles in tumor therapies is prospective.

关 键 词：纳米粒 聚乙烯亚胺 纳米复合物 溶酶体 质子化作用 生长作用 靶向作用 肿瘤病 细胞毒性 基因沉默 

分 类 号：R730.2[医药卫生—肿瘤]