p53β在氟尿嘧啶干预人胃癌细胞系中表达的生物学效应  

Biological effect of p53β on 5-FU treated human gastric cancer cell lines

作　　者：姜琪琪[1] 王莉 张红梅[3] 王燕泽 季万胜[3] 

机构地区：[1]山东省潍坊医学院,261053 [2]山东省潍坊市坊子区人民医院肿瘤科,261200 [3]山东省潍坊医学院附属医院消化内科,261031

出　　处：《中华消化病与影像杂志（电子版）》2015年第3期32-34,共3页Chinese Journal of Digestion and Medical Imageology(Electronic Edition)

基　　金：山东省优秀中青年科学家科研奖励基金(BS2010SW034)

摘　　要：目的探讨p53异构体p53β在氟尿嘧啶(5-FU)干预胃癌细胞系MKN45及SGC-7901中表达的生物学效应。方法将不同浓度的5-FU作用于MKN45和SGC-7901细胞内24h,采用细胞增殖/毒性检测试剂盒(CCK-8试剂盒)检测细胞抑制率;巢式逆转录多聚酶链反应(nRT-PCR)检测p53和P53βmRNA的表达变化;Western-blot检测p53和P53β蛋白的表达变化。结果不同浓度(25、50、100μg/ml)的5-FU对MKN45细胞有抑制作用,抑制率分别为18.204%、31.354%、52.969%,随药物浓度的增加而增加;对SGC-7901细胞抑制率分别为26.305%、26.363%、25.780%,虽有抑制作用,但与药物浓度无关;浓度为100μg/ml的5-FU作用24h后,对MKN45细胞抑制率明显高于SGC-7901细胞。nRT-PCR检测MKN45中,p53和P53βmRNA的表达随5-FU浓度的增加而增加。Western-blot检测MKN45中,p53和P53β蛋白的表达随5-FU浓度的增加而增加。结论 5-FU对表达野生型p53的人胃癌细胞MKN45的抑制效应中,随5-FU浓度的增加,上调p53、p53β的表达,推测p53β在肿瘤生长抑制实验中发挥着重要的作用。Objective To explore the expression of p53βand its biological effects in fluorouracil（5-FU） treated MKN45 and SGC-7901 gastric cancer cell lines.Methods MKN45 and SGC7901 cells were treated with 5-FU of different concentrations for 24 h.The growth inhibition rate was detected by CCK-8,and mRNA expressions of p53 and p53βwere measured by nested reverse transcription PCR （ nRT-PCR ） , and protein expressions of p53 and p53βwere measured by Western-blot.Results CCK-8 assay showed that the different concentrations of 5-FU（25,50 and 100μg/ml） had inhibiting effect on MNK45 cells,and the inhibition rates were 18.204%,31.354%,52.969%,which increased with the drug concentration increase. However,in SGC-7901 cells the inhibition rates were 26.305%,26.363%,25.780%, having statistically significance but not being associated with the drug concentration.After 24 h,the inhibition rate of 100μg/ml 5-FU in MKN45 was higher than that in SGS-7901.nRT-PCR and Western blot results showed that the expressions of p53 and p53βincreased with the increasing concentrations of 5-FU in MKN45 cells. Conclusions In the induction of 5-FU to the proliferation inhibition of MKN45 cells expressed wild？ type p53,the up-regulation of p53βassociates with wide-type p53,which infers that p53βplays an important role in the inhibition effect of gastric cancer cell growth.

关 键 词：肿瘤抑制蛋白质P53 氟尿嘧啶 胃肿瘤 细胞系 肿瘤 

分 类 号：R735.2[医药卫生—肿瘤]