P38MAPK特异性抑制剂SB203580对肝癌细胞增殖的影响  

Effect of P38MAPK Specific Inhibitor SB203580 on Proliferation of Hepatocellular Carcinoma

作　　者：邱建武[1] 李宏发[1] 张华献[1] 申丽娟[1] 

机构地区：[1]昆明医科大学基础医学院病理学教研室,云南昆明650500

出　　处：《昆明医科大学学报》2015年第9期8-12,共5页Journal of Kunming Medical University

基　　金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(2011FB242)

摘　　要：目的探讨P38MAPK通路对肝癌细胞增殖的影响.方法描绘生长曲线、MTT、台盼兰染色、共聚焦显微镜、Western Blot和原位细胞凋亡检测.研究正常肝细胞、癌周肝硬化细胞和肝癌细胞3株细胞在P38MAPK通路阻断前后增殖情况变化.结果 P38MAPK抑制剂对肝癌细胞的增殖影响最大,随着抑制剂浓度增加,增殖越明显.而对癌周肝细胞和正常肝细胞表现为低浓度促进增殖,高浓度抑制增殖.结论阻断P38MAPK的活化可以促进肝癌细胞的增殖.Objective To study the effect of P38 MAP kinase in proliferation of the human hepatocellular carcinoma （HCC） cell line. Methods The changes of cell proliferation was determined by depicting cell growth curve, MTI＇, trypan blue stain assays, confocal microscopy, Western Blot and TUNEL. The changes of proliferation situation in the normal liver cell, paratumor cirrhosis cell and HCC cell were investigated before and after the block by SB203580. Results P38MAPK specific inhibitor played an important role in the proliferation of HCC cell line QGY-7703, and the inhibitory effect was dose-dependent. But normal liver cell line HL-7702 and the paratumor cirrhosis cell line QSG-7701 displayed earlier spread and later restrain when concentration of SB203580 was increased. Gonclusion The block of P38MAP kinase plays an essential role in promoting HCC cell proliferation.

关 键 词：P38MAPK SB203580 肝细胞癌 

分 类 号：R735.7[医药卫生—肿瘤]