A型肉毒毒素ELISA鉴别试验方法的建立  

Establishment of an ELISA identification for botulinum toxin type A

作　　者：萧在澜 王媛[1] 顾磊[2] 杨雨生[1] 赵建荣[2] 徐永浩[1] 张华捷[2] 

机构地区：[1]兰州生物制品研究所有限责任公司甘肃省疫苗工程技术研究中心,甘肃兰州730046 [2]中国食品药品检定研究院,北京100050

出　　处：《微生物学免疫学进展》2015年第4期31-33,共3页Progress In Microbiology and Immunology

摘　　要：目的建立鉴定A型肉毒毒素的ELISA鉴别试验方法以替代传统的动物试验法。方法采用现代免疫学技术,制备马源性和兔源性抗A型肉毒毒素多克隆抗体,建立了双抗体夹心ELISA,并就此初步进行方法学验证。结果所建立的ELISA具有良好的特异性、灵敏度、精密度和耐用性,具有替代动物试验方法的良好前景。结论在进一步验证和确认之后,该方法有望正式成为可用于鉴定A型肉毒毒素的试验方法以替代动物试验法。Objective To establish a new identification method for botulinum toxin type A（ bot A） to replace the traditional animal test. Methods A double antibody sandwich ELISA was established based on the modern immunological technology,by using prepared two kinds of anti-bot A polyclonal antibodies derived from equine and rabbit origin,and a preliminary verification test was performed. Results The established identification ELISA could be a potential alternative method in replacement of animal test,it is of good specificity,sensitivity,precision and robustness. Conclusion The established method will be expected to be approved for the identification test of botulinum toxin type A as the replacement method for traditional animal test by a further verification and validation.

关 键 词：注射用A型肉毒毒素 鉴别试验 酶联免疫吸附分析 替代 动物试验法 

分 类 号：R392-33[医药卫生—免疫学]