贝母辛对LPS致黏液高分泌小鼠MUC5AC和EGFR表达的影响  

The effect of peimisine on LPS induced expression of MUC5AC and EGFR in mice with mucus hypersecretion

作　　者：崔艳茹 刘海云 屈飞[2] 

机构地区：[1]江西中医学院基础医学院生理学教研室,南昌330004 [2]江西中医学院药学院药理学教研室,南昌330004

出　　处：《中药药理与临床》2015年第3期17-21,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：江西省卫生厅中医药科研基金课题;2012A151;江西中医药大学重点学科基金和江西中医药大学道地药材专项基金

摘　　要：目的:探讨脂多糖致黏液高分泌小鼠MUC5AC表达的影响,并初步探讨其机制。方法:用乙醚麻醉小鼠,经鼻腔滴注脂多糖(1 mg/5 ml磷酸缓冲盐溶液,每只50μl),构建小鼠黏液高分泌模型。将小鼠随机分为对照组、脂多糖组和2mg/kg、4mg/kg、6mg/kg剂量贝母辛组。HE染色观察各组小鼠肺组织病理学改变、实时定量PCR法检测MUC5AC mRNA表达、免疫组织化学法检测MUC5AC蛋白表达及表皮生长因子受体蛋白表达。结果:脂多糖组肺组织病理学显示:气道上皮脱落,气道内有粘液分泌,肺泡结构呈实变,有大量炎症细胞渗出,贝母辛治疗组上述变化明显减轻,且具有剂量依赖性。贝母辛(2mg/kg、4mg/kg、6mg/kg)均可降低脂多糖致黏液高分泌小鼠MUC5AC mRNA表达,与脂多糖组相比,均有显著的统计学意义,具有剂量依赖性。免疫组织化学法检测MUC5AC蛋白,正常小鼠气道上皮仅有少量的MUC5AC蛋白表达,模型组明显高于空白组,贝母辛可下调脂多糖诱导的MUC5AC表达,具有剂量依赖性。贝母辛可下调脂多糖诱导的表皮生长因子受体蛋白表达,具有剂量依赖性。结论:贝母辛可降低脂多糖致黏液高分泌小鼠MUC5AC表达,其机制可能与表皮生长因子受体表达下调有关。Objective： To investigate the effects of Peimisine on MUCSAC expression of lipopolysaccharide （lipopolysaccharide, LPS） induced mu- cus hypersecretion in mice and study its mechanism. Method： Mice were anesthetized with ether, via intranasal administration of LPS （ 1 rag/5 ml PBS, each only 50td）, establishment of mouse mucus hypersecretion. The mice were randomly allocated into sham, LPS and Pei- misine groups. The lung tissues of mice were observed by HE staining, MUCSAC mRNA was observed by RT-PCR and immunohistochemical method was used to detect MUCSAC protein and EGFR protein expression. Results： Lung tissue showed serious damage and mucus hyperse- cretion in mice induced by LPS. We also found high expression of MUC.SAC mRNA （VS control, P 〈 0.01 ）, MUCSAC protein and EGFR protein in LPS group. Peimisine relieved lung damage and mucus hypersecretion induced by LPS, deregulated MUCSAC mRNA （VS LPS group,P 〈 0.01 ）, lowered MUCSAC protein and EGFR protein expression in a dose dependent manner. Conclusion： Peimisine lowered MUCSAC expression probably via deregulation of EGFR.

关 键 词：贝母辛 黏液高分泌 MUC5AC EGFR 

分 类 号：R285.5[医药卫生—中药学]