番茄红素对大鼠肝脏局部缺血再灌注损伤的保护作用  

The protection and mechanism of lycopene against focal hepatic ischemia-reperfusion injury in rats

作　　者：史欣[1] 刘书花[1] 焦清海[1] 武向丽[2] 

机构地区：[1]邯郸市第一医院重症医学科,邯郸056002 [2]邯郸市中心医院药学部,邯郸056001

出　　处：《中药药理与临床》2015年第3期64-66,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：邯郸市第一医院重症医学科研究基金

摘　　要：目的:研究番茄红素(Lycopene,LP)对大鼠肝脏局部缺血再灌注损伤的保护作用,并探讨其作用机制。方法:通过夹闭肝门静脉和肝动脉60min后去夹恢复血流的方法制作肝脏局部缺血再灌注模型,选取96只模型大鼠随机分为肝缺血再灌注模型对照组、番茄红素(5、10、20、40mg/kg)治疗组,复方丹参滴丸(135mg/kg)阳性对照组,并另取16只同龄大鼠作为假手术组;术后灌胃给药治疗4周,每天一次。4周后,通过生化分析仪测定各组大鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)活性,并通过紫外-可见分光光度计测定血清中丙二醛(MDA)含量以及总抗氧化能力(T-AOC)水平;检测肝脏组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性;并通过HE染色观察肝脏组织病理学改变。结果:较缺血再灌注模型对照组,番茄红素(10、20、40mg/kg)治疗组大鼠血清中ALT、AST活性和MDA含量显著降低;番茄红素(20、40mg/kg)治疗组大鼠血清中ALP活性显著降低,T-AOC水平显著升高,肝脏组织中SOD和CAT活性显著升高;番茄红素(40mg/kg)治疗组大鼠肝脏组织中GSH-Px活性显著升高;番茄红素各治疗组肝组织病理性改变出现不同程度的改善,其中以番茄红素40mg/kg治疗组效果最为显著。结论:番茄红素对大鼠肝脏缺血再灌注损伤具有剂量依赖性的保护作用,其作用可能与番茄红素能够有效改善抗氧化酶活性、抑制氧化应激损伤、改善肝功能有关。Objective： To investigate the protection and mechanism of lycopene against focal hepatic ischemia-reperfusion injury in rats. Methods： 96 focal hepatic isehemia-reperfusion rat models were randomly devided into six groups： hepatic ischemia-reperfusion control group, LP （5, 10, 20 and 40 mg/kg） treated groups and Fufangdanshendiwan（ 135 mg/kg） treated group; and selected another 16 same-aged rats as sham operation group. The drugs were given by intragastrie administration for 4 months, once a day. The activity of ALT, AST, ALP and the con- tent of MDA in serum were determined, and the level of T-AOC was also determined; the activity of SOD, GSH-Px, CAT in hepar were de- termined; and the histopathological changes of the hepatic tissue observed by HE staining. Results： Compared with the hepatic ischemia- reperfusion control group, the activity of ALT, AST and the content of MDA in the serum of LP （ 10, 20 and 40 mg/kg） treated groups were significantly decreased（ P 〈 0.05, P 〈 0.01 ） ; the activity of ALP in serum of LP （20 and 40 mg/kg） treated groups were significantly de- creased（P 〈0.05, P 〈0.01 ）, the level of T-AOC were signifieandy increased（ P 〈 0.05, P 〈 0.01 ）, the activity of SOD, CAT in hepar were significantly increased（ P 〈0.05, P 〈0.01 ） ; the activity of GSH-Px in hepar of LP 40 mg/kg treated group was significantly increased （ P 〈 0.01 ） ; the hepatic tissue histopathologieal changes of LP groups were significantly improved, especially the LP 40 mg/kg group. Con- clusion： LP had dose-dependent protective effects against hepatic isehemia-reperfusion injury in rats, which perhaps related to its effects of enhanceing the activity of antioxidant enzymes, redueeing the oxidative stress and improveing hepatic function.

关 键 词：番茄红素 肝脏 缺血再灌注 

分 类 号：R285.5[医药卫生—中药学]