趋化因子受体CXCR2参与头颈部鳞癌侵袭转移机制的实验研究  

The role of CXCR2 in the invasion and metastasis of head and neck squamous cell carcinoma

作　　者：王宗耀 纪东 刘建伟[2] 钱永[2] 

机构地区：[1]江苏省邳州市东大医院口腔科,221300 [2]南昌大学第一附属医院口腔颌面外科

出　　处：《实用口腔医学杂志》2015年第4期478-483,共6页Journal of Practical Stomatology

基　　金：国家自然科学基金(编号:81060087;81460410)

摘　　要：目的:观察及探讨趋化因子受体CXCR2在头颈部鳞癌(HNSCC)侵袭转移过程中的作用机制。方法:免疫组化检测105例HNSCC中CXCR2的表达水平,分析CXCR2表达与颈淋巴结转移的相关性;用RNAi技术,建立CXCR2基因沉默稳定表达的HNSCC细胞株,体外细胞迁移及侵袭试验观察CXCR2基因沉默对细胞迁移及侵袭能力的影响。结果:CXCR2在HNSCC中的阳性表达率为51.43%;其中,颈部淋巴结转移组为67.24%,无颈部淋巴结转移组为31.91%(P<0.05)。划痕试验及体外侵袭试验结果显示:CXCR2基因沉默的HNSCC细胞株体外迁移及侵袭能力明显下降(P<0.05)。结论:CXCR2可能在HNSCC的侵袭转移机制中发挥重要作用。Objective： To explore the role of CXCR2 in the invasion and metastasis of head and neck squamous cell carcinoma （HNSCC）. Methods： The expression of CXCR2 in HNSCC tissues of 105 cases was detected by immunohistochemical staining, the correlation between CXCR2 expression and cervical lymph node metastases of HNSCC was analysed. Then, 3 stable HNSCC cell lines with CXCR2 interference were established, the effects of CXCR2 silencing on cell migration and invasion were observed by in vitro tests. Results： CXCR2 was positively expressed in 51.43% of HNSCC specimens and was statistically associated with the cervical lymph, node metastases of HNSCC. CXCR2 silencing markedly inhibited the migration and invasion of HNSCC cells in vitro. Conclu- sion： CXCR2 may play a key role in the invasion and metastases of HNSCC.

关 键 词：头颈部鳞癌(HNSCC) CXCR2 侵袭 转移 

分 类 号：R739.91[医药卫生—肿瘤]