荧光原位杂交技术在膀胱癌诊断中的临床研究  

The study on fluorescence in situ hybridization technique in the diagnosis of bladder cancer

作　　者：汪亮亮[1] 周海滨[2] 童娟[3] 李婷[1] 张小容[1] 陈伟芳[1] 钱坤[1] 徐晓[1] 

机构地区：[1]九江学院附属医院/临床医学院病理科,江西九江332000 [2]九江学院附属医院/临床医学院泌尿外科,江西九江332000 [3]九江市妇幼保健院,江西九江332000

出　　处：《赣南医学院学报》2015年第3期365-368,共4页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：九江市科技计划项目基金(九科字[2013年]38号编号8)

摘　　要：目的:评价尿脱落细胞荧光原位杂交(FISH)检测在膀胱癌的诊断价值。方法:采用FISH检测68例血尿患者尿脱落细胞3、7、17号染色体和9号染色体上p16基因位点数目异常情况,以组织病理确诊为膀胱癌为金标准,评估FISH诊断的特异度和敏感度,并与尿细胞学检查结果做比较。结果:FISH诊断膀胱癌的总的敏感性高于尿脱落细胞学检查(分别为79.8%、24.4%,P<0.05),两者的特异性分别为93.4%、100%(P>0.05)。结论:FISH在诊断膀胱癌中敏感性、特异性高于尿细胞学检查,在早期膀胱癌诊断和膀胱癌术后复发的监测具有重要意义。Objective： To analyze the diagnostic value of fluorescent in situ hybridization to detect bladder cancer. Meth- ods ：The number of pl6 gene locus on chromosome 3,7,17 and 9 were examined with fluorescent in situ hybridization in 68 case of urinary exfoliative cell. To compare the sensitivity and specificity of the urine cytology test and fluorescent in Situ hybridization base on histopathological diagnosis. Results ： The sensitivity of fluorescent in situ hybridization is higher than urine cytology（ 79.8% vs 24.4% ,P 〈 0.05 ）. The specificity is 93.4% vs 100% （ P 〉 0.05 ）. Conclusion ： The sensitivity and specificity of FISH is greater than urine cytology test in the diagnosis of bladder cancer and have great significance in the early diagnosis and recurrence monitoring of bladder cancer.

关 键 词：膀胱尿路上皮癌 荧光原位杂交(FISH) 细胞诊断学 细胞块 

分 类 号：R730.43[医药卫生—肿瘤]