C-erbB-2、p-Akt蛋白表达水平与新辅助化疗治疗乳腺癌效果的关系研究  

STUDY ON THE RELATIONSHIP BETWEEN THE EXPRESSION OF PROTEIN LEVEL OF C-ERBB-2,P-AKT AND THE EFFECT OF NEOADJUVANT CHEMOTHERAPY FOR BREAST CANCER

作　　者：黄慧云[1] 黄兵 

机构地区：[1]西安医学院第一附属医院肿瘤科,陕西省西安市710077 [2]陕西省大荔县疾病预防控制中心

出　　处：《中国煤炭工业医学杂志》2015年第8期1259-1263,共5页Chinese Journal of Coal Industry Medicine

基　　金：国家自然科学基金项目(编号:No30772752)

摘　　要：目的探讨行新辅助化疗乳腺癌患者疗效与C-erbB-2、p-Akt蛋白表达的关系。方法选取该院2013年1月—2015年4月收治的67例乳腺癌新辅助化疗联合手术治疗患者为研究对象,采用免疫组织化学法检测C-erbB-2、p-Akt蛋白的表达以及原位末端转移酶标记法检测肿瘤细胞凋亡指数(AI),分析C-erbB-2、p-Akt蛋白表达与AI间的关系。结果化疗前的C-erbB-2、p-Akt蛋白表达情况与患者年龄、绝经、肿瘤直径、组织学分级、TNM分期均无相关性(P>0.05),有淋巴结转移CerbB-2、p-Akt蛋白表达阳性率显著高于非转移性患者(P<0.05)。CEF方案和TF方案间化疗有效率及AI值差异均无统计学意义(P>0.05)。化疗前,患者C-erbB-2、p-Akt蛋白表达情况与术后肿瘤细胞凋亡指标AI值均有明显呈负相关性(P<0.05)。结论化疗前若患者C-erbB-2、p-Akt蛋白呈现高表达,极有可能会降低乳腺癌患者对药物敏感性,对有效发挥术前新辅助化疗的治疗产生负面影响。Objective To investigate the relationship between the efficacy of neoadjuvant chemotherapy and C-erbB-2,the phosphorylation of protein kinase B（p-Akt）protein expression in breast cancer patients.Methods In 67 cases of breast cancer with neoadjuvant chemotherapy in author＇s hospital from January 2013 to April 2015 were treated with operation as the research subjects,respectively,immunohistochemical method was used to detect C-erbB-2,p-Akt protein expression,TDT mediated dUTP nick end labeling assay of tumor tissue of tumor cell apoptosis index（AI）,correlation analysis of AI and expression of C-erbB-2 and the p-Akt protein was performed.Results Expression of C-erbB-2 and pAkt before chemotherapy and patient age,menopause,tumor size,histological grade and TNM stage were not significant correlation（P〉0.05）,lymph node metastasis in patients with C-erbB-2,p-Akt protein expression positive rate was significantly higher than that of non- metastatic patients（P〈0.05）.By means of CEF,TE in patients with total effective rate difference was not statistically significant（P〉0.05）,and two chemotherapy results in patients with AI values with significant difference（P〈0.05）.Before chemotherapy and after operation,expression of C-erbB-2,p-Akt protein and AI showed a significant negative correlation（both P〈0.05）.Conclusion The high expression of C-erbB-2 and p-Akt protein after chemotherapy may reduce the drug sensitivity of breast cancer patients,so as to reduce the effect of preoperative neoadjuvant chemotherapy.

关 键 词：新辅助化学治疗 乳腺癌 C-ERBB-2 P-AKT 肿瘤细胞凋亡指数 

分 类 号：R737.9[医药卫生—肿瘤]