流体剪切应力对人骨肉瘤MG-63细胞分化相关基因表达的影响  

Effect of flow shear stress on the expression of genes related to differentiation in MG-63 cells

作　　者：张连昌[1] 李东韬[1,2] 赵学武[1,3] 曹新生[1] 张舒[1] 

机构地区：[1]第四军医大学航空航天医学教育部重点实验室 [2]海军总医院心脏中心 [3]解放军93175部队医院

出　　处：《西北国防医学杂志》2015年第7期421-424,共4页Medical Journal of National Defending Forces in Northwest China

基　　金：国家自然科学基金资助项目(31170889;30870595);教育部留学回国启动基金资助项目(HG4406)

摘　　要：目的:观察人骨肉瘤MG-63成骨细胞在流体剪切应力作用后,分化相关基因表达谱的变化特点。方法:MG-63细胞常规培养72h,随机分为剪切应力作用组和无剪切应力作用对照组。将剪切应力作用组的细胞载玻片置于流体剪切系统中进行实验,流体剪切应力设为1.5Pa,作用时间为60min。无剪切应力作用组的细胞静置相同时间。之后分别提取两组的RNA,用Affymetrix公司的全人类基因组芯片进行杂交,并对获得的数据进行分析。结果:MG-63细胞分化相关基因表达谱发生改变,234个与分化相关的基因出现差异表达,183个基因表达上调(Fold Change>2,P<0.05),51个基因表达下调(Fold Change<-2,P<0.05)。结论:流体剪切应力可以通过改变成骨细胞分化相关基因的表达,调控成骨细胞分化功能。Objective.To observe the expression changes of genes related to differentiation after exposing to fluid shear stress（FSS）in human osteosarcoma MG- 63 cells.Methods. MG- 63 cells were cultured for 72 h.Then MG-63 cells were randomly divided into the fluid shear stress group and the control group.The MG-63 cells in fluid shear stress group were exposed to 1.5 Pa FSS for 60 min, while the MG-63 cells in control group were treated with no FSS. Then the RNA was extracted from each group, the chip was scanned with Affymetrix gene chip and analyzed for differentially expressed genes.Results.. A total of 234 genes related to differentiation had a statistical significant change in the FSS group compared with those of the control group.Of the 234 genes, 183 genes showed up-regulated （Fold Change〉2, P〈0.05）, while the rest of them were down-regulated （Fold Changed〈-2, P 〈0.05）.Conclusion. The FSS has an effect on the differentiation of MG-63 cells via regulating the expression of genes related to cell differentiation.

关 键 词：基因芯片 剪切应力 MG-63 分化 

分 类 号：R738.1[医药卫生—肿瘤]