补肾健骨方对假体松动炎性因子IL-1、IL-6、TNF-α抑制的实验研究  

Study of Bushenjiangu Decoction on the Secretion of IL-1,IL-6,and TNF-αin Aseptic Loosening of Prostheses

作　　者：秦立武[1] 王友强[1] 谭训香[1] 姜红江[1] 江和训[1] 宋修刚[1] 

机构地区：[1]山东省文登整骨医院,山东文登264400

出　　处：《中国中医骨伤科杂志》2015年第8期11-13,共3页Chinese Journal of Traditional Medical Traumatology & Orthopedics

摘　　要：目的:观察中药补肾健骨方对致关节假体无菌性松动炎性因子IL-1,IL-6,TNF-α抑制作用。方法:人工髋关节翻修术中取出的假体界膜组织作为实验标本,将其放入RPMI培养液中培养,分空白对照组、低浓度补肾健骨方组(5%)、高浓度补肾健骨方组(20%),培养72h,取上清液,酶联法测定IL-1,IL-6,TNF-α.结果:与对照组相比,5%、20%补肾健骨方组均能显著抑制界膜细胞分泌IL-1,IL-6,TNF-α(P<0.05)。结论:补肾健骨方能显著抑制假体周围界膜细胞分泌IL-1,IL-6,TNF-α.Objective：To observe the effect of Bushenjiangu decoction on the secretion of IL-1,IL-6,and TNF-α in aseptic loosening of prostheses. Methods： The periprosthetic membrane organization was taken through the revision hip arthroplasty,which were cultured in RPMI-1640 medium. They were divided into control group,low concentration of Bushenjiangu decoction group （5 %）, and high concentration of Bushenjiangu decoction group （20 %）. The concentration of IL-1, IL-6,and TNF-α was measured using ELISA methods 72 h after cultivation. Results： The secretion of IL-1, IL-6, and TNF-α were all significantly inhibited in both low and high concentration of Bushenjiangu decoction group compared to the control group （P,0.05）. Conclusion：Bushenjiangu decoction is effective for inhibiting the secretion of IL-1, IL-6, and TNF-α in the interface membrane of prostheses.

关 键 词：补肾健骨方 IL-1 IL-6 TNF-Α 假体无菌性松动 

分 类 号：R-33[医药卫生]