过氧化物酶体增殖物激活受体γ基因rs3856806位点多态性与非酒精性脂肪性肝病的相关性分析  

Association between PPARγ gene rs3856806 polymorphism and nonalcoholic fatty liver disease

作　　者：许新[1,2] 姜曼 李江文[1,2] 董全勇 林中华[2] 初蕾蕾[2] 辛永宁[2,3] 宣世英[2,3,4] 

机构地区：[1]大连医科大学,辽宁大连116044 [2]青岛市市立医院消化内二科,山东青岛266021 [3]青岛市消化疾病重点实验室,山东青岛266021 [4]中国海洋大学医药学院,山东青岛266003

出　　处：《临床肝胆病杂志》2015年第7期1088-1091,共4页Journal of Clinical Hepatology

基　　金：青岛市民生科技计划项目(14-2-3-17-nsh)

摘　　要：目的探讨过氧化物酶体增殖物激活受体（PPAR）γ基因rs3856806位点多态性与非酒精性脂肪性肝病（NAFLD）的相关性。方法随机收集2013年5月-2014年5月收治的189例经B超证实为NAFLD患者和184例对照组健康者的血液标本,应用多重高温连接酶检测反应技术（i MLDR）检测PPARγ基因rs3856806位点基因型。计数资料及计量资料分别采用Pearsonχ2检验和t检验进行比较,Logistic回归分析用于计算比值比（OR）及其95%可信区间（95%CI）。结果 NAFLD组体质量、腰围、臀围、ALT、AST、GGT、空腹血糖（FPG）、总胆固醇（TC）、甘油三酯（TG）及低密度脂蛋白（LDL）均高于正常对照组,差异均有统计学意义（P值均〈0.05）;高密度脂蛋白（HDL）低于对照组,差异亦有统计学意义（P值均〈0.01）。NAFLD组与对照组两者rs3856806位点的等位基因及基因型分布频率差异无统计学意义（P值均〉0.05）;等位基因T携带者患NAFLD的风险是等位基因C的1.068倍（OR=1.068,95%CI：0.748~1.525,P〉0.05）。非条件Logistic回归分析结果表明,与CC携带者相比,TT的基因型携带者的NAFLD发生风险高（OR=1.077,95%CI：0.706~1.643,P〉0.05）。结论 PPARγ基因rs3856806位点多态性与NAFLD的发生无明显相关性。Objective To investigate the association between PPARγ gene rs3856806 polymorphism and nonalcoholic fatty liver disease（ NAFLD）. Methods The blood samples of 189 patients who were diagnosed with NAFLD by B- ultrasound and 184 healthy normal subjects were collected to determine the genotypes of PPARγ gene rs3856806 by improved multiple ligase detection reaction. Comparison of categorical data between the two groups was made by Pearson chi- square test,and comparison of continuous data was made by t test. And the logistic regression analysis was used for calculating odds ratio（ OR） and 95% confidence interval（ 95% CI）. Results The NAFLD group had significantly higher body weight,waist circumference,hip circumference,and levels of alanine aminotransferase,aspartate aminotransferase,gamma- glutamyl transpeptidase,glucose,triglyceride,total cholesterol,and low- density lipoprotein than the control group（ P 〈0. 05）. However,the NAFLD group had a significantly lower level of high- density lipoprotein than the control group（ P 〈0. 01）. There were no significant differences in the allele and genotype frequencies of rs3856806 between the two groups（ P〉 0. 05）. The patients with rs3856806 T allele showed higher risk of developing NAFLD than those with C allele（ OR = 1. 068,95% CI： 0. 748- 1. 525,P 〉0. 05）.The unconditional logistic regression analysis showed that the patients with TT / TC genotypes had higher risk of developing NAFLD than those with CC genotype（ OR = 1. 077,95% CI： 0. 706- 1. 643,P 〉0. 05）. Conclusion PPARγ gene rs3856806 polymorphism is not significantly associated with NAFLD.

关 键 词：脂肪肝 过氧化物酶体增殖物激活受体 多态性 单核苷酸 

分 类 号：R575.5[医药卫生—消化系统]