维生素C前体2-酮基-L-古龙酸二步混菌发酵研究新进展  

New Progress on Two-step Mixed Cultures Fermentation for 2-KGA Production of Vitamin C Precursor

作　　者：吕淑霞[1] 张云鹤[1] 白玲[1] 张林[1] 杨宇[1] 

机构地区：[1]沈阳农业大学生物科学技术学院,辽宁沈阳110866

出　　处：《微生物学杂志》2015年第3期1-8,共8页Journal of Microbiology

基　　金：国家自然科学基金(31370077);沈阳市科技计划项目(F13-218-9-00)

摘　　要：维生素C(Vc)二步混菌发酵是我国首创具有自主知识产权的唯一应用于工业化生产Vc的微生物转化方法,该方法利用混合菌发酵L-山梨糖生产Vc前体物质-2-酮基-L-古龙酸,再经化学转化合成Vc;具有简化工艺,减少污染,降低能耗等优点。本文主要从产酸菌代谢关键酶、伴生菌胞外物质、组学以及外源添加物等方面综述Vc二步混菌发酵的最新研究进展,并提出进一步研究和探索的方向。wo-step mixed cultures fermentation initiatively established with independent intellectual property rights is a microbial transformation method used in vitamin C industrial production in China. 2-keto-L-gulonic acid （2-KGA）- the precursor of Vitamin C is transformed from L-sorbose through the mixed cultures fermentation which has the advantages of simple operation, less pollution and lower energy consumption. In this paper, the latest new progresses of mixed cultures fermentation producing 2-KGA are reviewed from the aspects of key enzymes of Ketogulonicigenium vulgate, extracellular substances of co-helper, Omies and exogenous additives. Meanwhile the prospective research fields are also mentioned in order to provide some references for the researchers.

关 键 词：混菌发酵 伴生菌 普通生酮基古龙酸菌 L-山梨糖脱氢酶 2-酮基-L-古龙酸 

分 类 号：Q819[生物学—生物工程]