蛇葡萄素对大肠癌细胞株SW480增殖、凋亡的影响  

THE EFFECT OF AMPELOPSIN ON THE APOPTOSIS INDUCTION AND CELL GROWTH INHIBITION OF HUMAN COLON CANCER SW480 CELLS

作　　者：马海芝[1] 史振军[1] 杨振淮[1] 

机构地区：[1]广州市中医医院,广东广州510130

出　　处：《现代医院》2015年第8期12-14,共3页Modern Hospitals

基　　金：2013年广东省自筹经费类科技计划项目(编号:39)

摘　　要：目的研究蛇葡萄素在体外对人大肠癌细胞株SW480增殖及凋亡的影响。方法采用不同浓度的蛇葡萄素处理SW480细胞后,利用MTT法检测细胞活性,TUNEL法检测细胞凋亡,Western-blot检测Bcl-2蛋白水平。结果不同浓度的蛇葡萄素均能抑制SW480的细胞的增殖,低浓度与高、中浓度组对细胞增殖抑制情况有显著性差异(p<0.05),而中、高浓度组间无显著性差异(p>0.05)。低、中、高浓度蛇葡萄素均能有效诱导SW480细胞凋亡,低浓度与高、中浓度组对细胞诱导效果有显著性差异(p<0.05),而中、高浓度组间无显著性差异(p>0.05),Western-blot显示Bcl-xL的表达显著下调(p<0.05);Bax、Bid、Caspase-3、p-Capase-9及Caspase-9的表达显著上调(p<0.05)。结论蛇葡萄素能抑制结肠癌SW480细胞增殖,诱导其凋亡,其对凋亡的诱导作用主要是通过Bcl-2家族蛋白实现的。Objective To investigate the effect of ampelopsin on apoptosis induction and cell growth inhibition in Human Colon cancer SW480 cells in vitro.Methods Treated with ampelopsin at several concentrations, MTT and flow cytometry was used to detect the inhibition rate and apoptotic rate of SW480 cells.Western-blot was used to investigate expression of Bcl-2 family pro-tein.Results Significant difference of cell growth inhibition rate was observed among all groups after treated with ampelopsin （ p〈0.05）.Cell growth inhibition rate in the low -dosage group was significantly high than that in median and high -dosage group （p〈0.05）, while no marked difference was observed between the median and high groups （p〉0.05）.The similar results were observed in the experiment on apoptosis induction.Level of Bcl-xL was significantly up-regulated.Level of Bax, Bid, Caspase-3, p-Ca-pase-9 and Caspase-9 was significantly down-regulated after treatment of ampelopsin.Conclusion Ampelopsin can inhibit cell growth and induce apoptosis of SW480.Bcl-2 family protein might be involved in the progress.

关 键 词：蛇葡萄素 细胞株SW480 抑制增殖 细胞凋亡 

分 类 号：R735.350.5[医药卫生—肿瘤]