Evidence for novel age-dependent network structures as a putative primo vascular network in the dura mater of the rat brain  

作　　者：Ho-Sung Lee Dai-In Kang Seung Zhoo Yoon Yeon Hee Ryu Inhyung Lee Hoon-Gi Kim Byung-Cheon Lee Ki Bog Lee 

机构地区：[1]Ki Primo Research Laboratory, Division of Electrical Engineering, KAIST Institute for Information Technology Convergence, Korea Advanced Institute of Science and Technology (KAIST) [2]Nadi Primo Research Institute, Graduate School of Integrative Medicine, Sun Moon University [3]Pharmacopuncture Medical Research Center, Korean Pharmacopuncture Institute [4]Department of Anesthesiology and Pain Medicine, College of Medicine, Korea University [5]Acupuncture, Moxibustion & Meridian Research Group, Medical Research Division, Korea Institute of Oriental Medicine [6]Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University [7]Faculty of Liberal Education, Seoul National University [8]Korea Atomic Energy Research Institute

出　　处：《Neural Regeneration Research》2015年第7期1101-1106,共6页中国神经再生研究（英文版）

基　　金：supported by Global Ph.D.Fellowship Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Education(2014H1A2A1020588);by Korea Atomic Energy Research Institute,by a Korean Pharmacopuncture Foundation Grant funded by the Korean Pharmacopuncture Institute(KPI-2014-010);by the grant K13290 of KIOM and by Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Science,ICT and Future Planning(2013R1A1A2021577)

摘　　要：With chromium-hematoxylin staining, we found evidence for the existence of novel age-dependent network structures in the dura mater of rat brains. Under stereomicroscopy, we noticed that chromium-hematoxylin-stained threadlike structures, which were barely observable in 1-weekold rats, were networked in specific areas of the brain, for example, the lateral lobes and the cerebella, in 4-week-old rats. In 7-week-old rats, those structures were found to have become larger and better networked. With phase contrast microscopy, we found that in 1-week-old rats, chromium-hematoxylin-stained granules were scattered in the same areas of the brain in which the network structures would later be observed in the 4- and 7-week-old rats. Such age-dependent network structures were examined by using optical and transmission electron microscopy, and the following results were obtained. The scattered granules fused into networks with increasing age. Cross-sections of the age-dependent network structures demonstrated heavily-stained basophilic substructures. Transmission electron microscopy revealed the basophilic substructures to be clusters with high electron densities consisting of nanosized particles. We report these data as evidence for the existence of age-dependent network structures in the dura mater, we discuss their putative functions of age-dependent network structures beyond the general concept of the dura mater as a supporting matrix.With chromium-hematoxylin staining, we found evidence for the existence of novel age-dependent network structures in the dura mater of rat brains. Under stereomicroscopy, we noticed that chromium-hematoxylin-stained threadlike structures, which were barely observable in 1-weekold rats, were networked in specific areas of the brain, for example, the lateral lobes and the cerebella, in 4-week-old rats. In 7-week-old rats, those structures were found to have become larger and better networked. With phase contrast microscopy, we found that in 1-week-old rats, chromium-hematoxylin-stained granules were scattered in the same areas of the brain in which the network structures would later be observed in the 4- and 7-week-old rats. Such age-dependent network structures were examined by using optical and transmission electron microscopy, and the following results were obtained. The scattered granules fused into networks with increasing age. Cross-sections of the age-dependent network structures demonstrated heavily-stained basophilic substructures. Transmission electron microscopy revealed the basophilic substructures to be clusters with high electron densities consisting of nanosized particles. We report these data as evidence for the existence of age-dependent network structures in the dura mater, we discuss their putative functions of age-dependent network structures beyond the general concept of the dura mater as a supporting matrix.

关 键 词：nerve regeneration dura mater chromium-hematoxylin staining fascia primo vascular system brain hormone neural regeneration 

分 类 号：R742[医药卫生—神经病学与精神病学]