贵州地区汉族肺癌患者单核细胞趋化蛋白-1-2518A/G基因多态性的研究  

Study on the Relationship between Genetic MCP-1-2518 Polymorphism and Lung Cancer in Han Population of Guizhou Region

作　　者：金艳坤[1] 井晓婷[2] 罗靖[2] 余红[1] 张雯[2] 张湘燕[1] 叶贤伟[1] 

机构地区：[1]贵州省人民医院呼吸与危重症医学科,贵州省呼吸疾病研究所,贵州550002 [2]贵州医学院

出　　处：《医学研究杂志》2015年第7期62-65,共4页Journal of Medical Research

基　　金：国家科技部国家临床医学研究中心基金资助项目(2013BAI09B09);贵州省优秀科技教育人才省长专项基金资助项目[黔省专合字(2011)21号];贵州省呼吸疾病防治研究科技创新人才团队[黔科合人才团队(2012)4012号]

摘　　要：目的：研究贵州地区汉族人群中单核细胞趋化蛋白－1（MCP－1）－2518A／G位点是否存在基因多态性。探讨MCP－1－2518A／G基因多态性与肺癌的相关性。方法收集2013年3-12月在贵州省人民医院呼吸与危重症医学科住院治疗、无血缘关系的肺癌患者（实验组）90例及同期健康体检者（对照组）32例的血样。通过运用聚合酶链式反应－限制性片段长度多态性技术对MCP－1－2518A／G的基因型进行检测。结果贵州地区汉族人群中MCP－1－2518A／G存在基因多态性，健康人群中，3种基因型（ AA、AG、GG）的分布频率分别为：6．2％、46．9％、46．9％，实验组为26．7％、46．6％、26．7％。2．实验组和对照组中MCP－1－2518A／G 3种基因型分布差异具有统计学意义（P＝0．022），AA基因型个体患肺癌的相对风险度高（OR＝5.455，P＝0．015），GG基因型个体患肺癌的相对风险度低（OR ＝0．412，P＝0．035），A等位基因在实验组和对照组的分布频率差异具有统计学意义（P＝0．005），携带A等位基因的个体患肺癌的相对风险是携带G等位基因个体的2．368倍。结论贵州地区汉族人群中存在MCP－1－2518A／G基因多态性。 MCP－1－2518A／G基因多态性与肺癌的发病有相关性，A等位基因可能是肺癌发病的易感基因。Objective To study the gene polymorphism of monocyte chemotactic protein-1（MCP-1）-2518A/G in Chinese Han population of Guizhou region and the relationship between MCP-1-2518A/G gene polymorphism and lung cancer.Methods Totally 90 unrelated patients with lung cancer （22 with SCLC, 68 with NSCLC;20 in early stage,70 in late stage） in Department of Respiratory and Critical Care Medicine in the Guizhou Provincial People′s Hospital and 32 healthy individuals were recruited from March to December 2013.The gene polymorphism of MCP-1-2518G/A was detected with polymerase chain reaction-restriction fragment length polymor-phism（PCR-RFLP） in both patients with lung cancer and the control groups.Results （1）The gene polymorphism of MCP-1-2518 A/G existed in Chinese Han population of Guizhou region.In control group, the genotype frequency of AA, GG, AG were 6.2%, 46.9%, 46.9%.（2） There was a significant difference in genotype distribution between AA,AG,GG of MCP-1 -2518A/G in lung cancer patients compared with healthy individuals（P=0.022）.The relative risk of suffering from lung cancer in AA genotype of lung cancer patients was much higher （χ2 =5.868, OR=5.455, P=0.015）, and there was a significant decrease in frequency of the GG genotype （χ2 =4.433,OR=0.412, P=0.035）.The frequency distribution of A allele in the lung cancer group and the control group of the relative risk was significantly different （P=0.005）.The relative risk suffered from lung cancer of individuals with A allele was 2.368 folds which was higher than with G allele.Conclusion MCP-1 -2518A/G gene polymorphism exists in Chinese Han population of Guizhou region.MCP-1-2518A/G gene polymorphism is associated with the incidence of lung cancer.A allele may be one of lung cancer susceptibility genes.

关 键 词：单核细胞趋化蛋白-1(MCP-1) 基因多态性 肺癌 

分 类 号：R734[医药卫生—肿瘤]