GDNF通过AKT/β-catenin调节人胶质瘤细胞增殖的研究  

GDNF regulates the proliferation of glioma cells through AKT/β-catenin signaling pathway

作　　者：李周儒[1] 滕道辉 董国凯[1] 殷文江[1] 蔡红星[1] 

机构地区：[1]徐州医学院法医学教研室,江苏徐州221002 [2]江苏省徐州市铜山区公安局刑警大队,221000

出　　处：《重庆医学》2015年第22期3034-3036,共3页Chongqing medicine

基　　金：国家自然科学基金项目(81101899)

摘　　要：目的研究胶质细胞系源性神经营养因子(GDNF)促进人胶质瘤细胞增殖的机制。方法将胶质瘤样本分为低级别胶质瘤组和高级别胶质瘤组,脑挫裂伤患者样本作为正常对照组,每组各12例;将胶质瘤细胞系C6细胞分为细胞对照组、牛血清蛋白(BSA)组和GDNF组。CCK-8实验检测细胞增殖率,蛋白免疫印迹(Western blot)法检测各组AKT、p-AKT、β-catenin和p-β-catenin的表达。结果与正常对照组相比,胶质瘤组AKT、p-AKT、β-catenin和p-β-catenin的蛋白水平显著增加(P<0.05),且高级别胶质瘤组的蛋白水平明显高于低级别胶质瘤组(P<0.05)。CCK-8检测C6胶质瘤细胞实验中,与细胞对照组相比,GDNF组细胞增殖率明显增高(P<0.05),Akt表达水平没有明显变化,而p-Akt、β-catenin和p-β-catenin的蛋白表达均显著增加(P<0.05)。结论 GDNF可能通过上调胶质瘤细胞p-AKT、β-catenin和p-β-catenin来促进胶质瘤细胞的增殖。Objective To study the mechanism that glial cell Iine-derived neurotrophic factor （GDNF） promotes human glioma cells proliferation. Methods We divided glioma samples into two groups,including low-grade glioma group and high-grade glio- ma group,while cerebral contusion patients were treated as the control group, 12 cases in each group. C6 glioma cell lines were di- vided into three groups,such as GDNF group, BSA（bovine serum albumin） group and control group. CCK-8 （cell counting kit-8） was used to detect the cell proliferation,while Western blot was used to detect the expression of AKT, p-AKT,β-catenin and p-β- catenin in each group. Results Comparing with the control group,the expression levels of AKT, p-AKT,fl-catenin and p-β-catenin in glioma group had a significantly increased （P〈0.05）. Meanwhile,the high-grade gliomas group also had a significant increase in those more than low-grade gliomas group （P〈0.05）. CCK-8 test showed that the cell proliferation in GDNF group was significant- ly higher than the control group （P〈0.05）, and the expression levels of p-AKT, β-catenin and p-β-catenin proteins all had a signifi cant increase （P〈0.05）. However,the expression level of AKT had no obvious difference. Conclusion GDNF might promote the proliferation of glioma cells by up-regulating the expression of p-AKT,β-catenin and p-β-catenin.

关 键 词：神经胶质瘤 胶质细胞源性神经营养因子 AKT Β-CATENIN 

分 类 号：R739.41[医药卫生—肿瘤]