E-cadherin在口腔扁平苔藓中的表达及意义  

Expression and significance of E-cadherin in oral lichen planus

作　　者：杜勇[1] 李浩渤[1] 

机构地区：[1]河北医科大学第二医院口腔内科,石家庄市050000

出　　处：《河北医药》2015年第16期2426-2429,共4页Hebei Medical Journal

摘　　要：目的口腔扁平苔藓(OLP)是常见的口腔黏膜病变,部分OLP病例可发生癌变,E-cadherin是参与细胞间粘附连接的主要分子,在肿瘤的发生、发展及浸润转移中起到重要的作用,成为预示肿瘤进展及预后的重要指标之一。本实验的目的旨在检测E-cadherin在口腔扁平苔癣中的表达,探讨E-cadherin在OLP发病及癌变中的作用,为口腔扁平苔藓癌变的早期诊断和治疗提供依据。方法本研究使用52例口腔扁平苔藓标本蜡块和41例口腔正常黏膜标本蜡块。运用SP免疫组织化学法,以E-cadherin单克隆抗体作为一抗,用已知的E-cadherin阳性的正常口腔黏膜标本作为阳性对照,以PBS代替一抗作为阴性对照,对切片标本进行免疫组化染色,观察阳性染色的分布及强度,对表达情况进行研究分析。结果在52例口腔扁平苔藓标本中25例呈正常阳性表达,27例呈现异常阳性表达,异常阳性率为51.92%;在41例正常口腔黏膜标本中39例呈正常阳性表达,2例呈现异常阳性表达,异常阳性率为4.88%,2组比较差异有统计学意义。结论 E-cadherin在口腔扁平苔藓标本中的异常阳性表达率明显高于正常口腔黏膜,提示E-cadherin在口腔扁平苔藓恶变的过程中发挥着一定作用,进一步证实口腔扁平苔藓具有潜在恶变的可能。Objective To investigate the expression of E-cadherin in oral lichen planus （OLP） and to explore the role of E-cadherin in pathogenesis and carcinomatous change of OLP in order to provide scientific evidences for early diagnosis and treatment of OLP caneeration. Methods Fifty-two OLP specimens were collected, at the same time, 41 normal oral mucous membrane specimens were served as control group. Using E-cadherin monoclonal antibody as primary antibody, taking E-cadherin-positive normal oral mucous membrane specimen as positive controls and using PBS as negative controls instead of primary antibody, these specimens were detected by immmunohistology （SP） to observe the distribution and intensity of positive staining, moreover, the expression of E-cadherin was analyzed. Results Twenty-five specimens out of the 52 OLP specimens showed normal positive expression of E-cadherin, however, the other 27 specimens showed abnormal positive expression of E-eadherin, with abnormal positive rate being 51.92%. However in control group, 39 out of 41 specimens showed normal positive expression and 2 specimens showed abnormal positive expression, with abnomtal positive rate being 4.88%, there was a significant difference between two groups. Conclusion The abnormal positive expression rate of E-cadherin in OLP is significantly higher than that in normal oral mucous membrane, which suggests that E-cadherin plays a certain role in the canceration of OLP, and it is further confirmed that OLP is possible to be cancerated.

关 键 词：口腔扁平苔藓 E-钙粘蛋白 免疫组化 

分 类 号：R781.59[医药卫生—口腔医学]