瑞舒伐他汀影响大鼠模型脑缺血再灌注损伤选择素E和L表达的研究  

Expression of E-selectin and L-selectin affected by rosuvastatin in rats with cerebral ischemia reperfusion injury

作　　者：晁满香[1] 李建军[1] 吴小明[2] 赵钢[3] 

机构地区：[1]解放军第323医院神经内科,西安710054 [2]第四军医大学生物医学工程系,西安710032 [3]第四军医大学西京医院神经内科,西安710032

出　　处：《神经解剖学杂志》2015年第4期409-412,共4页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(30770670)

摘　　要：目的:探讨瑞舒伐他汀对脑缺血再灌注损伤大鼠脑组织中选择素E(E-selectin)和选择素L(L-selectin)表达的影响。方法:SD大鼠24只随机分为假手术组(sham)、模型组(model)和治疗组(test)。线栓法制作大鼠大脑中动脉脑缺血再灌注模型,于缺血2 h再灌注24 h后断头取脑,免疫组织化学法、RT-PCR法和Western Blot检测脑组织中E-选择素和L-选择素的表达量,结果进行统计学分析。结果:大鼠脑缺血再灌注损伤后,模型组选择素E和选择素表达明显高于假手术组(P<0.01);与模型组相比,治疗组选择素E和选择素表达明显下调(P<0.05)。结论:瑞舒伐他汀可能通过下调选择素E和选择素表达而对大鼠脑缺血再灌注损伤发挥保护作用。Objective： To explore expression of E-selectin and L-selectin affected by rosuvastatin in rats with the cere- bral ischemia-reperfusion. Methods： Rats were randomly assigned into three groups： sham operation group （sham group） , cerebral ischemia-reperfusion group （model group） and E-selectin and L-selectin group （test group）. The mid- dle cerebral artery occlusion reperfusion model was made by the suture method （ ischemia for 2 hours, and reperfusion for 24 hours）. The level of E-selectin and L-selectin expression was measured by immunohistochemistry, RT-PCR and West- ern Blot. Results： After eerebral ischemia reperfusion injuryin rats, compared with sham group, the expression of E-se- lectin and L-selectin were increased significantly in the model group （ P 〈 0.01 ）. Compared with model group, the expres- sion of E-selectin and L-selectin were decreased significantly in the test group （ P 〈 0.05 ）. Conclusion： Rosuvastatin can suppress the expression of E-selectin and L-selectin in cerebral isehemia-reperfusion.

关 键 词：瑞舒伐他汀 脑缺血再灌注 E-选择素 L-选择素 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]