消融稳斑胶囊对动脉粥样硬化大鼠血管内皮细胞AMPK蛋白及血清TNF-α、IL-6、CRP表达的影响  

Effect of Xiaorong Wenban Capsule on Vascular Endothelial Cells AMPK Protein,Serum TNF-α,IL-6,and CRP Expression

作　　者：孙晓泽[1] 邵静[2] 

机构地区：[1]河南省中医院,河南郑州450002 [2]河南中医学院第一附属医院,河南郑州450000

出　　处：《中医学报》2015年第7期993-995,共3页Acta Chinese Medicine

基　　金：河南省科技攻关课题(122102310079)

摘　　要：目的:观察消融稳斑胶囊对动脉粥样硬化大鼠血管内皮细胞AMP激活蛋白激酶(AMP-activated protein kinase,AMPK)蛋白表达及血清肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-6(interleukin-6,IL-6)、C反应蛋白(C-creactive protein,CRP)表达的影响,并探讨消融稳斑胶囊改善不稳定心绞痛的分子机制。方法:48只Wistar大鼠,随机分为空白组、模型组、瑞舒伐他汀组及消融稳斑胶囊组。观察大鼠给药前后IL-6、TNF-α、CRP等炎性因子的浓度,并采用Western blot方法测定大鼠血管内皮细胞的AMPK蛋白表达量。结果:消融稳斑胶囊能明显降低血清TNF-α、CRP水平,并与瑞舒伐他汀组比较,差异有统计学意义(P<0.05);消融稳斑胶囊能明显降低血清IL-6水平,并与瑞舒伐他汀效果相当;模型组AMPK表达明显高于空白组(P<0.01);与模型组比较,瑞舒伐他汀组、消融稳斑胶囊组能明显降低AMPK表达(P<0.01)。结论:消融稳斑胶囊可以降低动脉粥样硬化大鼠的血清炎症因子水平,同时增加血管内皮细胞AMPK蛋白表达。Objective：To observe the effect of Xiaorong Wenban Capsule on vascular endothelial cells AMPK protein expression,serum TNF-α,IL-6,and CRP expression and explore its molecular mechanisms in improving unstable angina pectoris.Methods：48 Wistar rats were randomly divided into blank group,model group,rosuvastatin group and Xiaorong Wenban group.Observed the IL-6,TNF-α,CRP, and expression of AMPK protein of rats before and after the administration and detect the vascular endothelial cell AMPK protein by western blot method.Results：Xiaorong Wenban Capsule can significantly decrease the levels of serum TNF-αand CRP,compared with Rosuvastatin group,the difference was statistically significant（P 〈0.05）.Xiaorong Wenban Capsule can significantly decrease the ser-um IL-6,correspondent to rosuvastatin group;model group AMPK expression was obviously higher than that of control group （P 〈0.01 ）.Compared with model group,rosuvastatin group,Xiaorong Wenban capsule group can significantly reduce AMPK expression （P 〈0.01 ）.Conclusion：Xiaorong Wenban Capsule can reduce the level of serum inflammatory factors in atherosclerotic rats,while in-creasing the expression of AMPK protein in vascular endothelial cells.

关 键 词：消融稳斑胶囊 动脉粥样硬化 AMP 激活蛋白激酶 肿瘤坏死因子-α 白细胞介素-6 C 反应蛋白 大鼠 TUMOR NECROSIS factor-α INTERLEUKIN 6 

分 类 号：R285.5[医药卫生—中药学]