小鼠肾脏急性缺血再灌注损伤不同时间点基因表达谱的差异  

Stage-Specific Differences in Gene Expression of Mouse Kidneys Insulted by Ischemia Reperfusion Injury

作　　者：黄凝[1] 仓静[1] 薛张纲[1] 王浩[1] 

机构地区：[1]复旦大学附属中山医院麻醉科,上海200032

出　　处：《中国临床医学》2015年第3期283-287,共5页Chinese Journal of Clinical Medicine

基　　金：国家自然科学基金项目(编号:81301615);上海市自然科学基金项目(编号:14ZR1406900)

摘　　要：目的:探讨小鼠肾脏急性缺血再灌注损伤不同时间点基因表达谱的差异。方法:建立小鼠肾脏急性缺血再灌注损伤模型,收集再灌注4 h与24 h时缺血侧与对照侧肾脏组织标本,采用基因表达谱芯片技术检测各组标本的基因表达水平,再结合生物信息学技术分析差异表达的基因种类(gene ontology,GO)与信号通路,采用实时荧光定量逆转录聚合酶链反应法验证表达上调、下调与未变化的基因。结果:小鼠肾脏急性缺血再灌注24 h差异表达的基因数目多于再灌注4 h;再灌注4 h时的变化基因主要为代谢与应激相关类型,24 h时的变化基因涉及炎性反应与细胞凋亡等;再灌注4 h和24 h时,缺血再灌注引起的表达上调的基因数目均多于表达下调的基因数目;随机挑选的表达上调、下调与未变化基因可以被实时荧光定量逆转录聚合酶链反应法验证。结论:缺血再灌注损伤伴随着大量基因表达的改变,缺血再灌注损伤不同时期的差异表达基因与病理生理变化密切相关。Objective：To analyze the stage-specific differences in gene expression of mouse kidneys insulted by ischemia reperfusion（IR）injury.Methods：Mouse kidney tissues were collected at 4 h and 24 h after IR.Total RNA of control and IR kidneys were prepared for Microarray and real-time fluorescence quantitative reverse transcription polymerase chain reaction（RT-qPCR）analysis.Results：The number of differential expressed genes（DEGs）at 24 h after IR was larger than that at 4 h.The DEGs at4 h were associated with metabolism and stress responses,while the DEGs at 24 h were associated with inflammation and apoptosis.The numbers of up-regulated genes at both 4 h and 24 h were larger than those of down-regulated genes.Several downregulated,up-regulated,or unchanged genes were confirmed by RT-qPCR.Conclusions：Stage-specific changes of gene expression occurres during IR injury.These DEGs at early or intermediate-late stages of IR injury are tightly associated with their stage-specific pathophysiological processes.

关 键 词：缺血再灌注 基因表达 小鼠肾脏 

分 类 号：R363[医药卫生—病理学]