不同规格毫针针刺对大鼠穴区筋膜组织、细胞和胶原纤维的影响  

Effects of acupuncture with different filiform needles on tissues,cells and collagenous fiber of fascia in acupoint area of rats

作　　者：李程[1,2] 陈波[1] 胡铁汉 陈磊[1] 

机构地区：[1]贵阳中医学院针灸推拿学院,贵州贵阳550025 [2]贵州省第二人民医院康复科,贵阳550002

出　　处：《中国针灸》2015年第8期801-805,共5页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金项目:81160456;贵州省高等学校特色重点实验室建设项目:黔教合KY字[2014]218号

摘　　要：目的:探讨不同规格毫针针刺对大鼠腧穴穴区筋膜结缔组织结构、细胞活性、胶原纤维排列形态及含量的影响。方法:将32只SD大鼠随机分为空白组、细针组、中针组和粗针组,每组8只。除空白组外,其他组分别采用不同规格毫针在大鼠"中脘"沿任脉循行方向平刺,施以捻转平补平泻刺激,每天1次,空白组予相同的麻醉、抓取、固定。干预3天后,切取各组穴区筋膜结缔组织,采用HE染色、增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)免疫组化染色和MASSON染色,镜下观察腧穴筋膜结缔组织形态、细胞中PCNA表达、胶原纤维的排列形态和表达变化。结果:各组毫针针刺后,腧穴筋膜结缔组织形态及胶原纤维排布一致性发生改变;各组腧穴筋膜结缔组织细胞中PCNA蛋白表达均明显增强(P<0.01,P<0.05);胶原纤维的面积分布发生改变,其中细针组较空白组增大,但差异无统计学意义(P>0.05),中针组与粗针组胶原纤维的面积分布均较空白组减小(均P<0.05)。结论:不同规格毫针针刺均能够改变受治腧穴穴区局部组织形态、增强细胞活性和调节胶原纤维蛋白表达变化,这可能是毫针针刺治疗"通经脉"作用的细胞生物力学原理之一,但规格不同,其所产生的刺激量不同,穴区组织细胞和胶原纤维变化的量-效差异存在复杂关系。Objective To explore the effects of acupuncture with different filiform needles on structure of fascial connective tissues,cellular activity,arrangement and content of collagen fibers in acupoint area of rats.Methods A total of 32 SD rats were randomly divided into a blank group,a thin needle group,a medium needle group and a thick needle group,8rats in each one.Except for the blank group,rats in the remaining groups were treated with horizontal acupuncture at＂Zhongwan＂（CV 12）towards Conception Vessel with different filiform needles,and twirling mild reinforcing-reducing method was applied,once a day.Rats in the blank group were treated with identical anesthesia,grasping and fixation.After 3-day intervention,the fascial connective tissue of acupoint area was collected.HE staining,immumohistochemical staining of proliferating cell nuclear antigen（PCNA）and MASSON staining were adopted to observe the morphology of fascial connective tissues,expression of PCNA in cells and arrangement and expression of collagenous fiber.Results After acupuncture in each group,the consistency of morphology of fascial connective tissues and arrangement of collagenous fiber were changed;the expression of PCNA protein in the fascial connective tissue in each group was significantly increased（P〈0.01,P〈0.05）.The area distribution of collagenous fiber were changed,and that in the thin needle group was insignificantly increased compared with that in the blank group（P〉0.05）,and that in the medium needle group and thick needle group were reduced compared with that in the blank group（both P〈0.05）.Conclusions Acupuncture with different filiform needles can change the local tissue morphology of acupoints,strengthen cell activity and adjust the expression of collagenous fiber protein,which may be one of the cellular biomechanics principles of the acupuncture therapy＇s＂regulating meridians＂effects.However,the stimulation is produced by different fifiform needles,and the complex relationships exist between cells

关 键 词：毫针针刺 筋膜结缔组织 形态结构 增殖细胞核抗原(PCNA) 胶原蛋白 

分 类 号：R224[医药卫生—中医基础理论]