深海链霉菌SCSIO 03032芳酰胺类代谢产物的研究  

Study on aromatic amide metabolites from the deep-sea-derived Streptomyces sp.SCSIO 03032

作　　者：聂方玲 陈玉婵[3] 张庆波[1] 田新朋[1] 章卫民[3] 张长生[1] 张文军[1] 

机构地区：[1]中国科学院热带海洋生物资源与生态重点实验室中国科学院海洋微生物中心广东省海洋药物重点实验室中国科学院南海海洋研究所,广东广州510301 [2]中国科学院大学,北京100049 [3]广东省微生物研究所,广东广州510070

出　　处：《中国海洋药物》2015年第4期49-55,共7页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(31125001;41106143;31470172)资助

摘　　要：目的对从印度洋深海沉积物中分离到的1株深海来源的放线菌Streptomyces sp.SCSIO 03032进行次级代谢产物分析及其活性研究。方法对深海来源放线菌Streptomyces sp.SCSIO 03032的发酵产物进行有机溶剂萃取,利用正、反向硅胶层析、硅胶柱层析、凝胶柱层析、薄层层析等分离手段进行纯化,通过ESIMS、1 H NMR、13 C NMR分析及文献查阅鉴定化合物结构,并对化合物进行抗菌及抗氧化活性研究。结果从深海来源放线菌Streptomyces sp.SCSIO 03032的发酵产物中分离得到3个芳酰胺类化合物,其结构分别鉴定为4-甲基苯-1,3-二氨基甲酸甲酯(1)、2-甲基苯-1,3-二氨基甲酸甲酯(2)、羰基亚氨基4-甲基-3,1-亚苯基双[氨基甲酸]甲酯(3);活性结果显示3个化合物均无明显的抑菌活性或抗氧化活性。结论得到了1株能够产生3个不同芳酰胺类化合物的深海链霉菌SCSIO 03032。Objective To investigate the bioactive secondary metabolites from the deep-sea-derived Streptomyces sp.SCSIO 03032 isolated from a sediment sample from Indian ocean.Methods The fermentation cultures of the Streptomyces sp.SCSIO 03032 were extracted with organic solvents and purified by reversed-phase MPLC,chromatography over silica gel,Sephadex LH-20,column preparative TLC（pTLC）.Their planar structures were elucidated by ESI-MS,1 H NMR,13 C NMR,comparison with those reported in literature and X-ray crystallographic analysis.The compounds were evaluated for antibacterial activities and antioxidative activity.Results Three aromatic amide metabolites were isolated from the fermentation broth and identified as 4-toluene-1,3-dicarbamic acid dimethyl ester（1）,2-toluene-1,3-dicarbamic acid dimethyl ester（2）,{carbonylbis imino（6-methyl-3,1-phenylene）]}bis [carbamic acid dimethyl ester（3）.Compounds 1~3 displayed no antibacterial activities or antioxidative activity against indicator or DPPH.Conclusion This work provided a marine-derived actinomycete Streptomyces sp.SCSIO 03032 which was able to produce three aromatic amide metabolites.

关 键 词：深海来源放线菌 STREPTOMYCES sp.SCSIO 03032 芳酰胺类化合物 

分 类 号：R931[医药卫生—生药学]