火箭煤油对小鼠细胞免疫的抑制作用观察  

Cell-mediated immune suppression effect of rocket kerosene through dermal exposure in mice

作　　者：徐冰心[1] 刘志国[1] 司少艳[1] 李成林[2] 娄晓同[1] 杨鹤鸣[2] 李建忠[1] 王建营 崔彦[2] 

机构地区：[1]解放军306医院特种医学实验研究中心,北京100101 [2]解放军306医院普通外科,北京100101 [3]总装备部防疫大队,北京100101

出　　处：《解放军医学杂志》2015年第8期671-676,共6页Medical Journal of Chinese People's Liberation Army

基　　金：解放军总装备部后勤科研项目(CZZ11C004)~~

摘　　要：目的观察皮肤涂抹火箭煤油(RK)对小鼠细胞免疫的抑制作用。方法建立皮肤迟发超敏反应(DTH)模型,观察皮肤火箭煤油的涂抹剂量与其细胞免疫功能抑制作用之间的关系。阴性对照和阳性对照组ICR雌性小鼠背部涂抹丙酮,火箭煤油组涂抹特定剂量及一定次数的火箭煤油(低剂量:0.5ml/kg;中剂量:1ml/kg;高剂量:2ml/kg),每组6只。通过测定耳肿胀度判定火箭煤油的免疫抑制作用。阳性对照组和各实验组采用1%二硝基氟苯(DNFB)致敏。1不同剂量RK染毒1次:分为阴性对照组、阳性对照组、火箭煤油低剂量组、火箭煤油中剂量组、火箭煤油高剂量组;2同一剂量RK不同染毒次数(1次/d,天数不同):分为阴性对照组、阳性对照组、低剂量RK 1次染毒组、低剂量RK 2次染毒组、低剂量RK 3次染毒组、低剂量RK 4次染毒组、低剂量RK 5次染毒组;3不同剂量RK 5次染毒(1次/d,连续5d):分为阴性对照组、阳性对照组、低剂量RK 5次染毒组、中剂量RK 5次染毒组、高剂量RK 5次染毒组。采用体外培养的淋巴细胞增殖活性实验等方法观察皮肤涂抹RK的细胞免疫抑制持续时间,MTT法观察刀豆蛋白A(Con A)诱导的淋巴细胞增殖活性,流式细胞仪检测外周血T淋巴细胞亚群(CD3+、CD4+和CD8+)和脾淋巴细胞细胞周期。结果 RK 1次染毒能降低DNFB致敏引起的ICR小鼠耳肿胀、脾脏系数升高及胸腺指数降低(P<0.05),RK对小鼠的细胞免疫抑制作用随剂量与染毒次数的增加而增加。RK 1次染毒可以降低Con A诱导的脾淋巴细胞增殖且持续至染毒后20d仍有显著差异(P<0.05),染毒后第10天CD4+/CD8+比值显著降低。结论皮肤染毒RK对小鼠细胞免疫具有明显的抑制效应。Objective To study the effect of cell-mediated immune suppression effect of rocket kerosene （RK） through dermal application in mice. Methods Skin delayed type hypersensitivity （DTH） was used to observe the relation of the RK amount the skin exposed and the cellular immune inhibitory function. Different amount of the undiluted fuel was smeared directly onto the dorsal skin of mice. Mice in negative and positive control groups were treated with acetone. After the last exposure, all the mice except those in negative control group were allergized by evenly smearing with 1% dinitrofluorobenzene （DNFB） solution on their dorsum, Five days after allergy, 1% DNFB solution was smeared onto right ear of all mice to stimulate the allergic reaction. Twenty-four hours after attack, the auricle swelling, spleen index and thymus index in corresponding mice were determined. In the first series of experiments, different dosages of RK were applied once, and the ICR mice were randomly divided into negative control group, positive control group and experimental group （0.5ml/kg.BW× 1, lml/kg.BW × 1 and 2ml/kg.BW × 1 group）. In the second series of experiments, the certain and same dosage of RK was applied for different times, and the ICR mice were randomly divided into negative control group, positive control group and experimental group （0.Sml/kg.BW× 1,0.SmL/kg.BW × 2, 0.Sml/kg.BW× 3, 0.Sml/kg.BW×4 and 0.SmL/kg.BW×5 group）. In the third series of experiments, the different dosages of RK were applied morethan once, and the ICR mice were randomly divided into negative control group, positive control group and experimental group （0.Sml/kg.BW×5, lml/kg.BW× 5 and 2ml/kg.BW× 5 group）. Lymphocyte proliferation experiment in vitro was conducted to observe the persistent time of the cell-mediated immune suppression in mice by ILK dermal exposure. The lymphocyte proliferation induced by concanavalin A （Con A） was analyzed by MTT assay, and T lymphocyte subsets （CD3＋, CD4＋ and CD8＋） in peripheral

关 键 词：火箭煤油 细胞免疫抑制 超敏反应 迟发型 淋巴细胞 

分 类 号：R858.3[医药卫生—航空、航天与航海医学]