高压氧预处理对模拟海拔4000m 急性暴露人体能量代谢的作用  

Effects of hyperbaric oxygen preconditioning on human energy metabolism when exposed to simulated 4 000 m acute hypoxia

作　　者：石路[1] 吴楠宁 张明月[3] 张延猛[1] 吴岩印[3] 周述尧[1] 刘洪涛[1] 李洋洋[1] 牟杰[3] 刘俊松[3] 

机构地区：[1]上海交通大学海洋水下工程科学研究院,上海200233 [2]解放军总装备部电子信息基础部技术基础局 [3]南京军区杭州疗养院海勤疗养区

出　　处：《中华航海医学与高气压医学杂志》2015年第3期169-172,共4页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

摘　　要：目的：探讨高压氧预处理（ hyperbaric oxygen preconditioning ，HBOP）对海拔4000 m急性暴露人体劳动时能量代谢和抗氧化酶的作用，为改善急性低氧环境下人体的劳动能力提供新的措施。方法对8名受试者进行3、5、7 d的HBOP，然后急性暴露于模拟4000 m高海拔，在HBOP前、后作运动负荷自身对比实验，在运动前、后检测血糖（ glucose ， Glu ）、血乳酸（ blood lactate ， BLA ）、乳酸脱氢酶（lactate dehydrogenase， LDH）、超氧化物歧化酶（superoxide dismutase， SOD）和谷胱甘肽（glutathione， GSH）水平。结果 HBOP后，人体在模拟4000 m体力负荷后的Glu含量维持在较高水平，差异无统计学意义（P＞0．05）；在4000 m低氧暴露下安静和体力负荷后的BLA含量[（3．3±0．8）、（6．7±2．0） mmol]均较海平对照值[（2．4±0．7）mmol]显著升高，差异有统计学意义（P＜0．05或P＜0．01）；HBOP 7 d后体力负荷后的BLA含量（4．5±1．3） mmol／L较4000 m对照值（6．7±2．0） mmol／L显著降低，同时LDH活力显著升高，差异均有统计学意义（P＜0．05）；经HBOP 3 d和7 d后体力负荷后的SOD活力较4000 m对照值有所升高，趋于海平对照值；经HBOP后安静和体力负荷后的GSH含量和海平对照值相近，差异均无统计学意义（ P＞0．05）；HBOP 3 d和7d比较，人体负荷后能量代谢变化差异无统计学意义（P＞0．05）。结论人体在急性高海拔低氧暴露前进行3 d的短期HBOP即可使体力负荷后的Glu含量维持在较高水平，LDH活性明显增加，加速机体对BLA的清除，使BLA含量显著降低，并能使机体抗氧化物能力维持在海平对照水平，对提高人体抗缺氧、抗疲劳和改善低氧下人体的劳动能力有积极作用。Objective To investigate the effects of hyperbaric oxygen preconditioning （ HBOP ） on human energy metabolism and antioxidant under acute hypoxia exposure , so as to provide new measures for the improvement of physical performance in those with acute hypoxia .Methods Eight healthy subjects had respectively 3-day, 5-day and 7-day HBOP, then, they underwent acute exposure to a simulated high altitude of 4 000 m.Exercise load experiments were conducted both before and after HBOP .Levels of glucose （ Glu）, blood lactate （ BLA） , lactate dehydrogenase （ LDH） and superoxide dismutase （ SOD） were detected both before and after physical exercises .Results Following HBOP , no significant changes could be noticed in Glu level in the subjects , who underwent simulated 4 000 m physical exercise and its level remained relatively high during exposure（P〉0.05）.During exposure to hypoxia at an altitude of 4 000 m, BLA level both at rest and under physical exercise[（3.3 ±0.8）、（6.7 ±2.0） mmol/L] elevated signficantly, as compared with that at the sea＆nbsp;level[（2.4 ±0.7）mmol/L], and statistical differences could be noted , when comparisons were made between them（P〈0.05 or P〈0.01）.Following 7-day HBOP, the BLA levels after physical load [（4.5 ±1.3）mmol/L] were significantly decreased , as compared with those detected at 4 000 m, and at the same time, LDH activity was obviously elevated, with statistical significance（P〈0.05）.As compared with that of the control value at 4 000 m[（6.7 ±2.0） mmol/L], LDH levels after 3-day and 7-day HBOP were increased to some extent, which were equivalent to the control value at the sea level .After HBOP, GSH levels both at rest and under physical exercise were almost the same as that of the sea level , without statistical signficance （P〈0.05）. And no statistical significace could be seen in the changes of nergy metabolism , when comparisons were made between them （P 〈0.05）.Conclusions Before acute hypoxia exposure, 3-day

关 键 词：高压氧预处理 急性高海拔暴露 体力负荷 能量代谢 抗氧化物 

分 类 号：R339.5[医药卫生—人体生理学]