肝细胞癌患者Treg细胞对CD8^+T淋巴细胞穿孔素表达的影响  

Effects of Treg cells of patients with hepatocellular carcinoma on perforin expression of CD8^+T lymphocytes

作　　者：张羽[1] 杨海超[1] 董天秀[1] 李甜甜[2] 王琦[3] 杨秀华[1] 

机构地区：[1]哈尔滨医科大学附属第一医院腹部超声科,150001 [2]齐齐哈尔市第一医院超声科 [3]大庆油田总医院超声科

出　　处：《肝脏》2015年第5期343-346,共4页Chinese Hepatology

基　　金：国家自然科学基金面上项目(81171346);黑龙江省教育厅科学技术研究面上项目(12511327)

摘　　要：目的研究肝细胞癌患者免疫抑制性Treg细胞对CD8+T淋巴细胞穿孔素表达的影响。方法采集20例肝细胞癌患者和20名健康人的外周血,用流式分析法检测抗-CD3/CD28刺激48 h后CD8+T淋巴细胞的穿孔素表达情况。免疫磁珠分离法分离健康人CD8+T淋巴细胞和肝细胞癌患者Treg细胞,一组CD8+T淋巴细胞单独培养,另一组CD8+T淋巴细胞与Treg细胞共同培养,用流式细胞法检测抗-CD3/CD28刺激48 h后两组CD8+T淋巴细胞穿孔素表达情况。结果肝细胞癌患者外周血中CD8+T淋巴细胞穿孔素表达量与健康人相近,分别为(10.74±3.96)%和(12.6±2.48)%,差异无统计学意义(P>0.05)。CD8+T淋巴细胞单独培养和与肝癌患者Treg细胞共培养后,健康人CD8+T淋巴细胞穿孔素表达量分别为(34.2±3.65)%和(20.43±4.52)%,差异有统计学意义(t=11.42,P<0.01)。结论肝癌患者免疫抑制性Treg细胞可使CD8+T淋巴细胞穿孔素表达量降低。Objective To investigate the influence of immunosuppressive regulatory T cells （Treg）in patients with hepatocellular carcinoma （HCC）on perforin expression of CD8 ＋ T lymphocytes.Methods Perforin expression of CD8 ＋ T lymphocytes in 20 healthy subjects and 20 HCC patients was detected by flow cytometry.CD8 ＋ T lymphocytes from 20 healthy subjects and Treg from 20 HCC patients were collected by magnetic activated cell sorting （MACS）,then were divided into two groups：single culture of CD8 ＋ T lymphocytes and co-culture of CD8 ＋ T lymphocytes and Treg.Both groups were stimulated by anti-CD3/CD28 for 48 hours.Perforin expressions of CD8 ＋ T lymphocytes in both groups were quantitatively detected by flow cytometry.Results Perforin expression of CD8 ＋ T lymphocytes in HCC patients （10.74%＆±3.96%）was similar with that in healthy subjects （12.6% ＆±2.48%）（P 〉0.05 ）.However,perforin expression was significantly decreased after CD8 ＋ T lymphocytes were co-cultured with Treg [（34.2%＆±3.65%）and （20.43%＆±4.52%）, respectively （P 〈0.01）].Conclusion Treg could significantly reduce perforin expression of CD8 ＋ T lymphocytes.

关 键 词：穿孔素 CD8+T淋巴细胞 TREG细胞 肝细胞癌 

分 类 号：R735.7[医药卫生—肿瘤]