大孔树脂纯化黄秋葵黄酮及其体外抗氧化活性研究  

Purification technology of flavone from Abelmoschus esculentus(L.)Moench with macroporous resins and research of the antioxidant ability in vitro

作　　者：刘爱敬[1] 廖争争 郭琳[1] 赵旭[1] 毕开顺[2] 贾英[1] 

机构地区：[1]沈阳药科大学中药学院沈阳市中药药效物质基础筛选与评价重点实验室,辽宁沈阳110016 [2]沈阳药科大学药学院中药质量控制关键技术国家地方联合工程实验室,辽宁沈阳110016

出　　处：《食品工业科技》2015年第16期284-288,共5页Science and Technology of Food Industry

基　　金：中药药效物质基础筛选与评价重点实验室(F13-287-1-00);阿尔茨海默病的诊断方法与治疗药物的筛选研究(2011412004-1);五味子镇静安神活性部位的开发研究(F12-153-9-00)

摘　　要：以黄秋葵为原料,以总黄酮为研究对象,开展了总黄酮提取、分离纯化及抗氧化活性的研究。采用静态和动态吸附-解吸实验对8种不同型号大孔树脂进行筛选,以吸附、解析效果为指标,考察大孔树脂纯化黄酮的工艺参数,并利用DPPH、ABTS、Fe3+法测定黄秋葵黄酮体外抗氧化活性。结果表明AB-8型大孔吸附树脂纯化效果最好,最佳工艺条件如下:上样液质量浓度为0.927 mg/m L,上样量为50 m L,用5 BV的30%乙醇作为解吸剂,以1.0 m L/min的洗脱速度进行解吸。抗氧化结果显示黄酮提取物对DPPH、ABTS自由基有明显的清除能力(IC50值分别为0.440 mg/m L和0.256 mg/m L),并对Fe3+表现出了较高的还原能力。In order to optimize purification technology of flavone from Abelmoschus esculentus（L.） Moench by macroporous resin,static and dynamic adsorption-desorption were used to select the best one from 8 different type macroporous resins. With the content of flavone as index,purification technology parameters of flavone were optimized. The flavone was evaluated by three models of antioxidant activity：DPPH- ,ABTS＋- ,Fe3＋. AB-8 type resin showed the best purifying profile, its optimum technology conditions were as follows;sample concentration was 0.927 mg/mL,sample volume was 50 mL,desorption rate was 1 mL/min,eluted by 5 BV 30% ethanol. And the flavone extracts had great antioxidant activity. The flavonoid extract exhibit obviously ability of scavenging DPPH,ABTS radical,and also showed a higher reduction capability. This optimized technology was simple and feasible with good purification effect,so it could provide a reference for development of related products of okra.

关 键 词：黄秋葵 黄酮 大孔树脂 最佳工艺 抗氧化 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]