脂肪组织来源干细胞恶性转化为恶性纤维组织细胞瘤  

Malignant Fibrous Histiocytoma from Malignant Transformation of Adipose-derived Stem Cells

作　　者：陈辉[1] 唐秋灵[2] 李文玉 林敏 杨立业 

机构地区：[1]汕头大学医学院第二附属医院神经外科,广东汕头515041 [2]汕头大学医学院第二附属医院儿科,广东汕头515041 [3]中心医院神经外科,广东潮州521021

出　　处：《汕头大学医学院学报》2015年第2期75-77,F0002,共4页Journal of Shantou University Medical College

基　　金：广东省社会发展计划资助项目(2013B022000021)

摘　　要：目的:探讨脂肪组织来源干细胞(ASCs)体外恶性转化及转化的细胞体内病理特征。方法:从Balb/C小鼠脂肪组织培养出ASCs,用3-甲基胆蒽(MCA)培养基处理第二代ASCs 1周,使其发生恶性转化,将转化的ASCs注入裸鼠皮下,肿瘤长出后取出肿瘤组织进行病理学检测。结果:经MCA处理1周后的ASCs,6个月后发生恶性转化,细胞成瘤后提示为恶性纤维组织细胞瘤(MFH)。结论:MFH的发生可能来源于ASCs的恶性转化。Objective：To study malignant transformation of adipose-derived stem cells（ASCs）in vitro and pathology character of transformed cell in vivo. Methods：Secondary passages of ASCs cultured from adipose tissue of Balb/C mice were treated with methylcholanthren（MCA）in culture medium for a week. The transformed cells were injected subcutaneously into immune-deficient mice,subsequently. The tumors were isolated from mice,and the pathobiology was done to testify the types of the tumor. Results：ASCs treated with MCA for a week,occurred malignant transformation after 6 months. The pathobiology testified the tumor was malignant fibrous histiocytoma（MFH）. Conclusion：MFH may originate from malignant transformation of ASCs.

关 键 词：恶性纤维组织细胞瘤 脂肪来源干细胞 恶性转化 肉瘤 

分 类 号：R733[医药卫生—肿瘤]